FastQCFastQC Report
Mon 10 Jul 2023
SRR8239840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10115427
Total Bases758.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC175449717.34476458581531RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA157156315.53629915968945RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAA5410825.349077206528207RNA PCR Primer, Index 1 (100% over 23bp)
AACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGA4908174.852162938845785RNA PCR Primer, Index 1 (100% over 22bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA2654082.623794329196385RNA PCR Primer, Index 1 (100% over 22bp)
AACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAAC1425921.4096488462622487RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT1321331.3062523213305774RNA PCR Primer, Index 1 (100% over 25bp)
CGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACT1281091.2664714994236033RNA PCR Primer, Index 1 (100% over 25bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGG1122091.1092858462623476Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA902120.8918259209423388RNA PCR Primer, Index 1 (100% over 32bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGCC778490.7696066611918607No Hit
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA645610.6382429530656492RNA PCR Primer, Index 3 (100% over 36bp)
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG611030.60405754497561RNA PCR Primer, Index 1 (100% over 29bp)
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGC574770.5682113073427351No Hit
TAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGG509070.503261009149688Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAAC449440.4443114462691491RNA PCR Primer, Index 1 (100% over 24bp)
AGAGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA444750.43967496379539883RNA PCR Primer, Index 3 (100% over 36bp)
ACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA337540.3336883356481145RNA PCR Primer, Index 1 (100% over 32bp)
ATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC311870.30831125566918727RNA PCR Primer, Index 1 (100% over 27bp)
ATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC308520.3049994824736514RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGC300250.2968238513312389No Hit
ATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC269820.26674108764760995RNA PCR Primer, Index 1 (100% over 26bp)
GCGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC251670.2487981970509006RNA PCR Primer, Index 1 (100% over 24bp)
CGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTC248470.2456347122074036RNA PCR Primer, Index 1 (100% over 26bp)
ACGAAGACTGACGCTCAGGTGCGTGGAATTCTCGGGTGCCAAGGAACTCC244960.24216476476969284RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC243380.24060279412821622RNA PCR Primer, Index 3 (100% over 42bp)
GATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTC241550.2387936762333414RNA PCR Primer, Index 1 (100% over 26bp)
ATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACG235090.23240739120553192No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGG230820.22818611611749062No Hit
TTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC225540.22296636612572063RNA PCR Primer, Index 3 (100% over 39bp)
TGCGTAGAGATCTGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC218600.21610555837138662RNA PCR Primer, Index 1 (100% over 33bp)
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAAC202550.2002387047032221No Hit
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA200420.19813301010426945RNA PCR Primer, Index 1 (100% over 23bp)
ATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGTGGAATTCT171630.16967153240293267No Hit
ACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAA170460.1685148832570291No Hit
AGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG163140.16127841167752976RNA PCR Primer, Index 3 (100% over 38bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAG162990.16113012332549084No Hit
AATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCTGGAAT149960.14824880847837665No Hit
ATGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC135980.13442833406834925RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG126910.12546183171506256Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA126200.12475993351541165RNA PCR Primer, Index 3 (100% over 36bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCAA115340.11402385682779381No Hit
AAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTG111010.109743266398937No Hit
AACGATGTCGACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT106290.10507712625477895RNA PCR Primer, Index 3 (100% over 34bp)
AGGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC104790.10359424273438976RNA PCR Primer, Index 1 (100% over 24bp)
AATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT101200.10004520817559161RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)