FastQCFastQC Report
Mon 10 Jul 2023
SRR8239841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11642376
Total Bases873.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC141448412.149444408941955RNA PCR Primer, Index 1 (100% over 24bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA10940159.396836178457043No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG10045178.628109932199408No Hit
AAGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAA6831255.867573766729403RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT3367482.8924336406932745No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATGGAATTCTCGGGTGCC1988621.7080877649029718No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCTG1880421.6151514089563848No Hit
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC1306291.1220132385348145RNA PCR Primer, Index 1 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC1133550.97364146287665RNA PCR Primer, Index 1 (100% over 27bp)
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT853240.7328744579285191RNA PCR Primer, Index 1 (100% over 25bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG718790.6173911579560736No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA673440.5784386279913998No Hit
AGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAAC665080.5712579631511643RNA PCR Primer, Index 1 (100% over 24bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGTGGAATTCTCGG617140.530080801375939No Hit
ACGGTGCTGAAGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT598420.5140016092934981RNA PCR Primer, Index 3 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT532580.4574495790206397RNA PCR Primer, Index 1 (100% over 25bp)
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG513230.44082926028157826RNA PCR Primer, Index 1 (100% over 29bp)
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA500420.42982635159695926RNA PCR Primer, Index 4 (100% over 36bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC484760.4163754889895327RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA483660.41543066466844913RNA PCR Primer, Index 1 (100% over 23bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT353960.30402728790068284No Hit
AAGGCTGAGGCGTGATGATGAGGCACTGGAATTCTCGGGTGCCAAGGAAC284180.2440910686959432RNA PCR Primer, Index 1 (100% over 24bp)
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCATGGAATTCTCGGGT276740.23770062056061408No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTT270560.23239242573852623No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCT262060.22509151053015297No Hit
AAATTGAAGAGTTTGATCATGGCTTGGAATTCTCGGGTGCCAAGGAACTC255680.21961152946786805RNA PCR Primer, Index 1 (100% over 26bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTTGGAATTCTCGGG243550.2091926939999189No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA225060.19331105609370458RNA PCR Primer, Index 1 (100% over 22bp)
ACGGTGCTGAAGCAACAAATGCCTGGAATTCTCGGGTGCCAAGGAACTCC219940.1889133283446609RNA PCR Primer, Index 1 (100% over 27bp)
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT217700.18698932245445432No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGTGGAATTCTCGGG169390.14549435613486458No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCC148760.12777460545854213No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTTGGAATTCTC132210.11355929408223889No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCTGGAATT131730.11314700710576606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128100.11002908684619017No Hit
GTAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAG125410.10771856191554026No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAA123310.10591480639347157No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA118680.10193795493291061No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGTGGAATTCTCGGGTG117390.10082993368363985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)