FastQCFastQC Report
Mon 10 Jul 2023
SRR8239842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15846758
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG8768355.533213796790486No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA8532275.384236952441629RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC8469775.344796708575975RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC7262934.583227686066765RNA PCR Primer, Index 1 (100% over 24bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA6657904.201427194130181No Hit
AAGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAA3483212.1980584293645427RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT2574871.6248560115576953No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGA2199091.3877223341203293RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAA2176791.3736500551090638RNA PCR Primer, Index 1 (100% over 23bp)
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCTG2089571.3186104059896668No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA1899411.1986110976137831RNA PCR Primer, Index 1 (100% over 22bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATGGAATTCTCGGGTGCC1629861.0285132138699915No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGCC1301500.8213036382583744No Hit
TGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1244630.7854161715601388RNA PCR Primer, Index 1 (100% over 32bp)
ACGGTGCTGAAGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1107250.6987233603239223RNA PCR Primer, Index 1 (100% over 34bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG1062320.6703705578137812No Hit
AACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAAC929180.5863533727214109RNA PCR Primer, Index 1 (100% over 24bp)
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGC907020.5723694398564046No Hit
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG891620.5626513637679076RNA PCR Primer, Index 1 (100% over 29bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT862530.5442942966630777RNA PCR Primer, Index 1 (100% over 25bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGTGGAATTCTCGG774130.48851001573949704No Hit
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA725300.457696142012139RNA PCR Primer, Index 5 (100% over 36bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC723320.45644667508647513RNA PCR Primer, Index 1 (100% over 27bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA668500.42185284838703285No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGG641340.40471369601277435Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACT565860.3570825023011016RNA PCR Primer, Index 1 (100% over 25bp)
ACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCAC519510.32783361745033274No Hit
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC459020.2896617718273984RNA PCR Primer, Index 1 (100% over 26bp)
ACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA453520.2861910303672209RNA PCR Primer, Index 1 (100% over 32bp)
ATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC453190.2859827858796102RNA PCR Primer, Index 1 (100% over 31bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGG442430.2791927534956992Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGTGCTGAAGCAACAAATGCCTGGAATTCTCGGGTGCCAAGGAACTCC397710.2509724702049466RNA PCR Primer, Index 1 (100% over 27bp)
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT373680.2358084852434801RNA PCR Primer, Index 1 (100% over 25bp)
ACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAA373180.23549296329255487No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT355120.22409631042513553No Hit
TGCGTAGAGATCTGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC351310.22169203315908526RNA PCR Primer, Index 1 (100% over 33bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCAA350550.2212124397936789No Hit
AGAGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA340260.2147189980436377RNA PCR Primer, Index 5 (100% over 36bp)
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAAC316190.19952977132609712No Hit
AATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGC306580.19346543942931418No Hit
AGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAAC302330.19078350284644974RNA PCR Primer, Index 1 (100% over 24bp)
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGT288220.18187947339133975No Hit
AAGGCTGAGGCGTGATGATGAGGCACTGGAATTCTCGGGTGCCAAGGAAC262120.16540922755304271RNA PCR Primer, Index 1 (100% over 24bp)
ATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGTGGAATTCT260610.16445635126124852No Hit
ACGGTGCTGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG257780.1626704970190117RNA PCR Primer, Index 5 (100% over 37bp)
AGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA234940.1482574543007472RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTG234620.14805552025215504No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCT231490.1460803528393631No Hit
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCATGGAATTCTCGGGT221620.13985194952809907No Hit
ACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA209510.13221000787669RNA PCR Primer, Index 1 (100% over 32bp)
ATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC207870.13117509587765522RNA PCR Primer, Index 1 (100% over 27bp)
AAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTG203520.12843005490460574No Hit
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC191780.12102159949688132RNA PCR Primer, Index 1 (100% over 33bp)
TTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG191340.12074394018006711RNA PCR Primer, Index 5 (100% over 39bp)
ATTCATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG191240.12068083578988208RNA PCR Primer, Index 1 (100% over 29bp)
AGGCACGCTGATATGTAGGTGAAGCGACTTGCTCGTGGAGCTGAAATCAG188660.1190527425231079No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGTGGAATTCTCGGG180570.11394759735713765No Hit
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT179650.11336703696743523RNA PCR Primer, Index 1 (100% over 25bp)
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCTGGAATTCTCGGGTG179500.11327238038215766No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCATCCCGACT179440.11323451774804665No Hit
ATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACG179370.11319034467491709No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGG178200.11245202330975206No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCC176400.11131614428642124No Hit
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCA173680.10959970487338798No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCA170750.10775074624096613No Hit
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAA169020.10665904029076483No Hit
ACGAAGACTGACGCTCAGGTGCGTGGAATTCTCGGGTGCCAAGGAACTCC168270.10618575736437699RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166710.10520132887749027No Hit
ACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAA166290.10493629043871308No Hit
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA165270.1042926256588256RNA PCR Primer, Index 1 (100% over 23bp)
CGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAAC163110.10292957083082861RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCATGGAATTCTCGGG160330.10117526878368434No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA160270.1011374061495733No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCATGGAATTCTCGGGTGCC159290.10051898312575987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)