FastQCFastQC Report
Mon 10 Jul 2023
SRR8239843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292603
Total Bases546.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC73039610.015573314494153RNA PCR Primer, Index 1 (100% over 24bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG4115995.644061523711081No Hit
AAGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAA4080365.595203797601488RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA3191014.375680398343363No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC2586703.5470188079619858RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA1860732.5515306400197573RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT1346411.8462680609379118No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCTG1120901.5370369126085706No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATGGAATTCTCGGGTGCC891851.2229515304754695No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAA618290.8478317001487672RNA PCR Primer, Index 1 (100% over 23bp)
AACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGA593570.813934338671665RNA PCR Primer, Index 1 (100% over 22bp)
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG568700.7798312893215221RNA PCR Primer, Index 1 (100% over 29bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTGGAATTCTCG504570.6918928673342015No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA434740.5961383061713356RNA PCR Primer, Index 1 (100% over 22bp)
AGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAAC410700.563173396385351RNA PCR Primer, Index 1 (100% over 24bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA395850.5428102969543248No Hit
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT372810.5112166396552781RNA PCR Primer, Index 1 (100% over 25bp)
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC331630.4547484622431798RNA PCR Primer, Index 41 (100% over 36bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGCC323270.44328479145237987No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGTGGAATTCTCGG292840.40155757827486294No Hit
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC272010.3729943889719487RNA PCR Primer, Index 1 (100% over 27bp)
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGC257720.35339919093360767No Hit
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC232990.31948811693163603RNA PCR Primer, Index 1 (100% over 26bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGACTGGAATTCTCGGGTGCCA218390.2994678306223443No Hit
ACGGTGCTGAAGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG217390.2980965781354065RNA PCR Primer, Index 6 (100% over 34bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCTGGAATTCT207970.28517937970845253No Hit
CGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACT196700.2697253641806636RNA PCR Primer, Index 1 (100% over 25bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT196530.2694922512578842RNA PCR Primer, Index 1 (100% over 25bp)
ACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAA195690.26834039916885644No Hit
AAGGCTGAGGCGTGATGATGAGGCACTGGAATTCTCGGGTGCCAAGGAAC195380.2679153108979057RNA PCR Primer, Index 1 (100% over 24bp)
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAAC184730.25331147191201825No Hit
TGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA167990.2303567052806796RNA PCR Primer, Index 1 (100% over 32bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGG161880.22197835258548967Illumina Small RNA Adapter 2 (100% over 21bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGG152820.2095548050538333Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAAC143160.19630850603001423RNA PCR Primer, Index 1 (100% over 24bp)
ACTGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATC139790.19168738514903388RNA PCR Primer, Index 41 (100% over 42bp)
TTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGAC134000.18374783324966407RNA PCR Primer, Index 41 (100% over 39bp)
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCT127090.17427247856492395No Hit
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT120540.1652907747754814RNA PCR Primer, Index 1 (100% over 25bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGCTGGAATTCTCG107560.14749191749502888No Hit
ACGGTGCTGAAGCAACAAATGCCTGGAATTCTCGGGTGCCAAGGAACTCC101450.13911356479983897RNA PCR Primer, Index 1 (100% over 27bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCATGGAATTCTCGGGTGCC98370.13489010714007058No Hit
ATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC97540.1337519675759122RNA PCR Primer, Index 1 (100% over 27bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCC89440.12264482243171608No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGTGGTGGAATTCTCG87430.11988860493297113No Hit
ACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA85890.11777687610308692RNA PCR Primer, Index 1 (100% over 32bp)
ACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCAC82910.1136905436920123No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCAA79130.10850720929138746No Hit
TTGGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA77170.10581955441698938RNA PCR Primer, Index 41 (100% over 40bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC76590.10502422797456547RNA PCR Primer, Index 1 (100% over 33bp)
ACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTG74920.10273423632137936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)