FastQCFastQC Report
Tue 11 Jul 2023
SRR8246561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8246561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37409183
Total Bases920.1 Mbp
Sequences flagged as poor quality0
Sequence length10-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN15473384.136251786092201No Hit
AGGCTTGTAGCTC13222703.5346134129686817No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA5574471.4901341202773661No Hit
TGTGGGCACTCGAAGATACGG5552931.4843761757641165No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG4926201.3168424448082707No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG4891751.307633475983691No Hit
TGTGGGCACTCGAAGATACG4017681.0739822893218491No Hit
TGTGGGCACTCGAAGATA3970241.0613009110623988No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT3466300.9265906716006068No Hit
TGTGGGCACTCGAAGATACGGATTCT3358030.8976485800291334No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC2946510.7876435045373753No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC2758150.7372922311615306No Hit
TGTGGGCACTCGAAGATACGGATTCTT2631210.7033593863838191No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC2531210.6766279819583336TruSeq Adapter, Index 7 (100% over 50bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT2516970.6728214299681444No Hit
TGTGGGCACTCGAAGATACGGATTC2500980.6685470784005093No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC2297200.6140738224622547No Hit
ATTGAACTCGCTGTGAAG2262790.6048755461994452No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG2043630.546291000260551No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC2034440.5438343841938489No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA1952460.5219199788458357No Hit
GGGGCTATAGCTC1867570.4992276896290411No Hit
GCGAAGGTGGCGGAATTGGTAGACG1815750.48537547585575447No Hit
TCCTCTGTAGTTCAG1783130.4766556917321611No Hit
TGTGGGCACTCGAAGATACGGA1622740.4337811921741247No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT1556600.4161010412871086No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG1498830.4006583089505055No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG1494220.39942599120649064No Hit
TGTGGGCACTCGAAGATACGGAT1474660.3941973285008657No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1424370.38075410521528896No Hit
TGTGGGCACTCGAAGATACGGATTCTTA1423800.3806017362100637No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC1355510.36234686012789963No Hit
ATCTGAGGGTCGGACGTTCGAATCGTTCCGGGTGC1172250.3133588883777547No Hit
GCCCAGATGGCGGAATTGGTAGACGCACCAGCT1153380.30831467236266563No Hit
GGGGCTGATTCTGGATTCGACGG987420.2639512335781297No Hit
AGGAGTGTAGCTCAGTTGGTAGAGCGTCGGTCT973400.2602034906776767No Hit
TGTGGGCACTCGAAGATACGGATT968730.25895513409100646No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG959200.2564076312492577No Hit
CGGGACGTAGCGCAGCCTGGTAGCGCATCACACT943110.2521065482771971No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA932100.2491634206499511No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTA885600.2367333175921003No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC873640.23353624162281225No Hit
TGTGGGCACTCGAAGA844590.22577076863720866No Hit
GATTGTCTGATAGAAAGTG840350.22463735708956808No Hit
GCGAAGGTGGCGGAATTGGTAGACGC742770.19855285265117922No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACT732910.19591713617482637No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC727420.19444958207186722No Hit
GCCCAGATGGCGGAATTGGTAGACGCA695520.18592226406013732No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG682270.18238035297376048No Hit
GGGGCTGATTCTGGA678560.18138861786957497No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA673490.18003333566520285No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT672350.1797285976547523No Hit
GATTGTCTGATAGAAAGTGAAAAGCA659890.1763978646633368No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG577190.15429099320346024No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGA575250.1537724039576058No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT562820.15044969038751796No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG554680.14827375406728344No Hit
TCTGAGGGTCGGACGTTCGAATCGTTCCGGGTGC539130.1441170206791204No Hit
AATTGAACTCGCTGTGAAG537300.14362783597813403No Hit
TGTGGGCACTCGAAG507840.13575276423438598No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG496510.13272409611297847No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG489500.13085022466275192No Hit
TGTGGGCACTCGAAGATAC469810.12558681113137382No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG458110.122459236813592No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC443350.11851368152039034No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC437220.11687504642910806No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGT435890.11651951875024912No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA419890.11224249404217142No Hit
AGCGAAGCTCTTGATCG417350.11156351636976408No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT417300.11155015066755132No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC412390.11023763871025999No Hit
AGGGGCGTAGTTC404910.10823812965923367No Hit
AGGAGTGTAGCTCAGTTGGTAGAGCG399370.10675720985406176No Hit
TGTGGGCACTCGAAGATACGGAA396660.1060327887941311No Hit
GCGGGCGTAGCTCAGGGGTAGAGCA391090.10454384956763156No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACA384460.10277155745422187No Hit
ATTTATCAGACAATCTGAAA380910.10182259259711712No Hit
TGTGGGCACTCGA378970.10130400335126272No Hit
CTTTAACAATTTATCAGACAATCTGAAA374140.10001287651751176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)