FastQCFastQC Report
Tue 11 Jul 2023
SRR8246562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8246562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78036986
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA34257204.389867133002805No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC33504434.29340389953041No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG20793572.66457881907433No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG20440732.619364361406782No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG17989872.3053004635519883No Hit
NNNNNNNNNN17726852.2715959327286166No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC14460701.8530572157156353No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC14129061.8105594185813378No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG10950081.4031910458458763No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT10945741.4026348993027487No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC10141681.2995991413609953No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC10037741.2862798160861826No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC8877871.137649011713497No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA8417121.0786064956429764No Hit
AGGCTTGTAGCTC7242600.928098376326323No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT7106880.9107066231389306No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA7076750.9068456334282311No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT6959930.8918758087351042No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA6505120.8335944701913525No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA6163200.7897793489871584No Hit
GTGAGGTGTCCGAGTGGCTGAAGGAGC5529500.7085742650286365No Hit
TGTGGGCACTCGAAGATACGG5350400.6856236092972632No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG4638970.5944578638647063No Hit
ATTGAACTCGCTGTGAAG4542280.5820675852345194No Hit
TGTGGGCACTCGAAGATACG4345490.5568500556902595No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT4299340.5509361932558492No Hit
CCTCTGTAGTTCAGTCGGTAGAACGG4294240.550282656995492No Hit
CCTCTGTAGTTCAGTCGGTAGAACG4256380.5454311113450743No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT4041890.5179454265442799No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGGA3735000.4786192024381875No Hit
TGAGGTGTCCGAGTGGCTGAAGGAGC3362580.43089567810832674No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC3353320.4297090612905014No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT3276090.41981247199885446No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC3190620.4088599731414537No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT3138790.4022182507151161No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGAT2806110.3595871834414517No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC2552120.3270397962320072No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT2449680.3139126875043585No Hit
TGTGGGCACTCGAAGATACGGATTCT2433710.31186622199888653No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGC2393530.30671738142218874No Hit
TGTGGGCACTCGAAGATA2393120.3066648422326306No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC2379640.3049374561954507TruSeq Adapter, Index 8 (100% over 50bp)
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC2320880.29740769332121564No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC2091620.26802931625268045No Hit
GCGAAGGTGGCGGAATTGGTAGACGC2043480.26186044653236606No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT1996100.25578896652928135No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC1967990.25218682843542933No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGA1948950.24974695973009517No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACT1942070.2488653265004366No Hit
TGTGGGCACTCGAAGATACGGA1865300.23902768361658663No Hit
TGTGGGCACTCGAAGATACGGATTCTT1803050.2310506969092835No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC1798840.23051120913357673No Hit
GCGAAGGTGGCGGAATTGGTAGACG1768180.226582302909546No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCC1694290.21711371579625077No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGGAC1664400.21328348073309752No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG1588180.20351631724987432No Hit
TGTGGGCACTCGAAGATACGGATTC1572880.20155570846880222No Hit
TGTGGGCACTCGAAGATACGGAT1508920.19335959489773222No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGT1495540.19164502329702993No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGT1438280.18430747696995883No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC1428350.1830350034277336No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1417980.18170614636500698No Hit
GCCGAGGTGGTGGAATTGGTAGACACG1365960.17504007650936185No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG1357680.17397904116901697No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC1347170.17263224389522167No Hit
CTCTGTAGTTCAGTCGGTAGAACGG1239230.15880034116130523No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGC1230280.15765344909656046No Hit
GGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACT1214950.15568899598454505No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGTC1209550.1549970164147549No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGC1185970.15197537229333793No Hit
AATTGAACTCGCTGTGAAG1173120.15032871720596694No Hit
TGTGGGCACTCGAAGATACGGATTCTTA1142550.14641134397476602No Hit
TCCTCTGTAGTTCAG1138220.14585647887528613No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTG1119150.1434127658390087No Hit
CCGAGGTGGTGGAATTGGTAGACACG1097100.1405871825956989No Hit
GGGGCTATAGCTC1049590.13449904382519334No Hit
TCCTCTGTAGTTCAGTCGGTAGAAC1048590.13437089946041741No Hit
CGAGGTGGTGGAATTGGTAGACACG1046140.1340569457667163No Hit
CGGACGTGGGGGTTCAAGTCCCCCCC1020250.13073928816266686No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCC997200.12778556055458112No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGT988060.12661432106052892No Hit
GAAGGTGGCGGAATTGGTAGACGCG985620.1263016488104756No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG927710.1188808086463001No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAC919930.11788384548834319No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCT916140.1173981783458423No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG915400.11730335151590812No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG905860.11608085427594551No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGA879380.11268759149667826No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC872600.11181877270349729No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTA862160.11048094553523632No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCT860520.11027078877700378No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTCT810590.10387254064374041No Hit
GGCTATAGCTCAGTTGGTAGAGCCCTGGA810430.10385203754537625No Hit
GGGGCTGATTCTGGATTCGACGG801920.10276152900113288No Hit
GAGGTGTCCGAGTGGCTGAAGGAGC791390.10141216884004209No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG790960.10135706676318841No Hit
TGTGGGCACTCGAAGATACGGATT790630.10131477912281237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)