FastQCFastQC Report
Tue 4 Jul 2023
SRR8258332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8258332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5105532
Total Bases183.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAAT61141611.975559060250724No Hit
AATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCT1905493.732206555555817No Hit
AACGCGAAGAACCTTACCTACTCTTGACATCCAGAG1525002.986956109568993No Hit
ATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTC1173732.2989377013012553No Hit
TAATTGAAGAGTTTGATCATGGCTCAGATTGAACGC1093332.1414614578852897No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA1055592.0675416391474974RNA PCR Primer, Index 4 (100% over 36bp)
ACGCGAAGAACCTTACCTACTCTTGACATCCAGAGA952181.86499663502256No Hit
GCAACGCGAAGAACCTTACCTACTCTTGACATCCAG867311.6987651825510055No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAA848461.6618444463769886No Hit
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGA837941.6412393458703225No Hit
TGAGCCGTGGCTTTCGGAGCTAACGCGTTAAGTAGA837081.6395548984905002No Hit
CGCGAAGAACCTTACCTACTCTTGACATCCAGAGAA763751.4959263794644713No Hit
GTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATT656551.2859580549098506No Hit
CAACGCGAAGAACCTTACCTACTCTTGACATCCAGA465850.9124416417329282No Hit
CGAAGAACCTTACCTACTCTTGACATCCAGAGAATC390490.7648370434266204No Hit
TTGGAAACGATGGCTAATACCGCATGATGCCTACGG348570.6827300269589928No Hit
CGAAGAACCTTACCTACTCTTGACATCCAGAGAAGC322200.6310801695102489No Hit
ACGGTCGCAAGATTAAAACTCAAATGAATTGACGGG314470.615939729689286No Hit
GCGAAGAACCTTACCTACTCTTGACATCCAGAGAAT279180.5468186273242436No Hit
ATTTGACGTTAGCAACAGAAGAAGCACCGGCTAACT274130.5369273956171462No Hit
AAGAACCTTACCTACTCTTGACATCCAGAGAATCTA264340.5177521167235852No Hit
GGCAACGCGAAGAACCTTACCTACTCTTGACATCCA261020.5112493663735728No Hit
GTGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATG258890.5070774211188961No Hit
GCGAAGAACCTTACCTACTCTTGACATCCAGAGAAG237200.46459409127197715No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAA226800.4442240299345886No Hit
CGAGGCAACGCGAAGAACCTTACCTACTCTTGACAT214930.42097473877354996No Hit
CGATGCAACGCGAAGAACCTTACCTACTCTTGACAT207030.4055013267961106No Hit
AAGAACCTTACCTACTCTTGACATCCAGAGAAGCCA205760.40301382892125637No Hit
GGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGG197090.38603224894095267No Hit
AGTACGGTCGCAAGATTAAAACTCAAATGAATTGAC189520.37120519467902663No Hit
CGGTCGCAAGATTAAAACTCAAATGAATTGACGGGG184490.36135313616680886No Hit
TCGATGCAACGCGAAGAACCTTACCTACTCTTGACA183650.35970786198186594No Hit
TCGAGGCAACGCGAAGAACCTTACCTACTCTTGACA181140.35479162602447695No Hit
TGCAACGCGAAGAACCTTACCTACTCTTGACATCCA170890.33471536364868537No Hit
AGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATGG170620.3341865255178109No Hit
AGGCAACGCGAAGAACCTTACCTACTCTTGACATCC170550.3340494193357323No Hit
TTTGACGTTAGCAACAGAAGAAGCACCGGCTAACTC168430.3298970606784954No Hit
GGGGCTGATTTAGGATTCGACGGGTGGAATTCTCGG163910.3210439186357073No Hit
ATGCAACGCGAAGAACCTTACCTACTCTTGACATCC161880.31706783935542854No Hit
ATACCCTGGTAGTCCACGCTGTAAACGATGTCTACT154450.30251499745765964No Hit
GTACGGTCGCAAGATTAAAACTCAAATGAATTGACG147140.28819719472916827No Hit
AGCCGTGGCTTTCGGAGCTAACGCGTTAAGTAGACC145320.2846324339951253No Hit
ATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCT144960.2839273164872926No Hit
GGGGCTGATTTAGGATTCGACGGGAATTTTGCAGTC135430.26526128912716634No Hit
TGCTTGGCGACCATAGCGTTTTGGACCCACCTGAAT124410.24367685874851047No Hit
GAGGCAACGCGAAGAACCTTACCTACTCTTGACATC115310.22585305507829548No Hit
ACGGTCGCAAGATTAAAACTCAAATGAATTCTCGGG113500.22230788094169227No Hit
GAAGAACCTTACCTACTCTTGACATCCAGAGAATCT111520.21842973464861254No Hit
AGCAACGCGAAGAACCTTACCTACTCTTGACATCCA111110.21762668415358086No Hit
GATGCAACGCGAAGAACCTTACCTACTCTTGACATC110880.21717619241246552No Hit
TACGGTCGCAAGATTAAAACTCAAATGAATTGACGG108690.21288672757315005No Hit
GAAGAACCTTACCTACTCTTGACATCCAGAGAAGCC105020.2056984463127447No Hit
CGAAGCAACGCGAAGAACCTTACCTACTCTTGACAT103280.20229037835822009No Hit
AATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTC96700.18940239724283384No Hit
GTAGACCGCCTGGGGAGTACGGTCGCAAGATTAAAA95280.18662110040638272No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCAGTAAT94910.18589639630111024No Hit
TTAATTGAAGAGTTTGATCATGGCTCAGATTGAACG94660.18540673136511532No Hit
ATTCGAGGCAACGCGAAGAACCTTACCTACTCTTGA90560.1773762264147987No Hit
ATTCGATGCAACGCGAAGAACCTTACCTACTCTTGA89500.17530004708618024No Hit
ACGGTCGCAAGATTAAAACTCAAATGGAATTCTCGG87160.17071678328526782No Hit
TCGAAGCAACGCGAAGAACCTTACCTACTCTTGACA84540.1655850947560411No Hit
TTCGAGGCAACGCGAAGAACCTTACCTACTCTTGAC83400.16335222264790428No Hit
GTACGGTCGCAAGATTAAAACTCAAATGAATTCTCG81780.1601791938626572No Hit
TTCGATGCAACGCGAAGAACCTTACCTACTCTTGAC81050.15874937224955205No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGAGTAA80390.15745665681852547No Hit
AAGCAACGCGAAGAACCTTACCTACTCTTGACATCC78200.15316719197921No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG77380.1515610909891467RNA PCR Primer, Index 1 (100% over 29bp)
AGTACGGTCGCAAGATTAAAACTCAAATGAATTCTC72380.14176779226924835No Hit
AGTACGAGAAGGACGGCCAGACGGTTGGAATTCTCG71210.13947616036879212No Hit
GTACGGTCGCAAGATTAAAACTCAAATGGAATTCTC69460.1360485058168277No Hit
ACCCTGGTAGTCCACGCTGTAAACGATGTCTACTTG69390.13591139963474913No Hit
TGTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGG62530.1224749937910486No Hit
CGGTCGCAAGATTAAAACTCAAATGAATTCTCGGGT60620.11873395368004744No Hit
AGTACGGTCGCAAGATTAAAACTCAAATGGAATTCT60560.11861643409540867No Hit
TCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGT60040.11759793102853924No Hit
GGAGTACGGTCGCAAGATTAAAACTCAAATGAATTG58610.1147970475946483No Hit
CTCGTGTCGGTGGTTCGATTCCGCCTCCGGGCACCA58130.11385689091753808No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGGTGGA57780.11317136000714519No Hit
TGGTCGGGTAGAGAATACCAAGGCGCTTGGAATTCT57270.11217244353771558No Hit
GAAGCAACGCGAAGAACCTTACCTACTCTTGACATC54910.10755000654192354No Hit
TCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGG54120.10600266534417961No Hit
AGTACGAGAGGACCGGAGTGGACGTGGAATTCTCGG53010.10382855302836218No Hit
TAACACATGCAAGTCGAGCGGCAGCACAGAGATGGA52180.10220286544085906No Hit
GACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGT52070.10198741286902129No Hit
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGG51950.10175237369974373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)