FastQCFastQC Report
Tue 4 Jul 2023
SRR8258333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8258333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834528
Total Bases210 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAAT72405812.409881313449862No Hit
AATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCT2589834.438799505289888No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA2311653.962017150316187RNA PCR Primer, Index 5 (100% over 36bp)
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAA1850973.172441712508707No Hit
ATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTC1690302.8970638241859494No Hit
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGA1688102.8932931678449396No Hit
AACGCGAAGAACCTTACCTACTCTTGACATCCAGAG1591612.727915608597645No Hit
ACGCGAAGAACCTTACCTACTCTTGACATCCAGAGA1145281.9629351337417524No Hit
TAATTGAAGAGTTTGATCATGGCTCAGATTGAACGC921511.5794079658200284No Hit
GCAACGCGAAGAACCTTACCTACTCTTGACATCCAG768321.3168503090567052No Hit
GTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATT692901.187585353948083No Hit
CGCGAAGAACCTTACCTACTCTTGACATCCAGAGAA683411.1713201136407263No Hit
TGAGCCGTGGCTTTCGGAGCTAACGCGTTAAGTAGA678981.1637273829176926No Hit
CAACGCGAAGAACCTTACCTACTCTTGACATCCAGA519120.8897377817023074No Hit
CGAAGAACCTTACCTACTCTTGACATCCAGAGAATC353340.6056016870602043No Hit
ATTTGACGTTAGCAACAGAAGAAGCACCGGCTAACT333750.5720257062782114No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAA310900.5328622983727218No Hit
ACGGTCGCAAGATTAAAACTCAAATGAATTGACGGG289030.4953785464736822No Hit
GTGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATG272510.4670643452220985No Hit
TTGGAAACGATGGCTAATACCGCATGATGCCTACGG271800.4658474515847726No Hit
CGAAGAACCTTACCTACTCTTGACATCCAGAGAAGC271550.4654189679096578No Hit
GCGAAGAACCTTACCTACTCTTGACATCCAGAGAAT266320.4564550894262569No Hit
GGGGCTGATTTAGGATTCGACGGGTGGAATTCTCGG260610.4466685222866357No Hit
GGCAACGCGAAGAACCTTACCTACTCTTGACATCCA249600.42779810123458145No Hit
AAGAACCTTACCTACTCTTGACATCCAGAGAATCTA230870.3956961042949832No Hit
CGAGGCAACGCGAAGAACCTTACCTACTCTTGACAT224870.3854124960922289No Hit
CGATGCAACGCGAAGAACCTTACCTACTCTTGACAT224240.3843327172309397No Hit
TCGATGCAACGCGAAGAACCTTACCTACTCTTGACA223930.3838013974737974No Hit
GGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGG223850.38366428269776065No Hit
TCGAGGCAACGCGAAGAACCTTACCTACTCTTGACA221680.3799450443977645No Hit
GCGAAGAACCTTACCTACTCTTGACATCCAGAGAAG215810.3698842477060698No Hit
ATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCT186820.3201972807397616No Hit
AGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATGG181230.3106163857641955No Hit
AGGCAACGCGAAGAACCTTACCTACTCTTGACATCC169660.29078616127988416No Hit
CGGTCGCAAGATTAAAACTCAAATGAATTGACGGGG167470.2870326442858788No Hit
ATACCCTGGTAGTCCACGCTGTAAACGATGTCTACT165750.28408467660108927No Hit
ATGCAACGCGAAGAACCTTACCTACTCTTGACATCC165180.2831077338218276No Hit
TGCAACGCGAAGAACCTTACCTACTCTTGACATCCA164880.28259355341168985No Hit
TTTGACGTTAGCAACAGAAGAAGCACCGGCTAACTC162800.279028569234735No Hit
AAGAACCTTACCTACTCTTGACATCCAGAGAAGCCA161040.2760120441619271No Hit
AGTACGGTCGCAAGATTAAAACTCAAATGAATTGAC159420.2732354699471834No Hit
GTACGGTCGCAAGATTAAAACTCAAATGAATTGACG144750.24809204789144898No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTTGGAATT138140.23676293952141458No Hit
ACGGTCGCAAGATTAAAACTCAAATGAATTCTCGGG136920.23467193918685453No Hit
TGTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGG132510.22711348715783006No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCAGTAAT130800.22418265882004507No Hit
AGCCGTGGCTTTCGGAGCTAACGCGTTAAGTAGACC125900.2157843787877957No Hit
GATGCAACGCGAAGAACCTTACCTACTCTTGACATC118310.20277561441131142No Hit
GAGGCAACGCGAAGAACCTTACCTACTCTTGACATC118160.20251852420624258No Hit
AATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTC118130.20246710616522878No Hit
CGAAGCAACGCGAAGAACCTTACCTACTCTTGACAT106610.18272257841594042No Hit
GGGGCTGATTTAGGATTCGACGGGAATTTTGCAGTC106540.18260260298690828No Hit
AGCAACGCGAAGAACCTTACCTACTCTTGACATCCA104390.1789176433809213No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGAGTAA102690.1760039543901409No Hit
ATTCGAGGCAACGCGAAGAACCTTACCTACTCTTGA102670.17596967569613173No Hit
ATTCGATGCAACGCGAAGAACCTTACCTACTCTTGA102440.17557547071502613No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCGGTGGA101600.17413576556664054No Hit
TCGAAGCAACGCGAAGAACCTTACCTACTCTTGACA99370.17031369118461684No Hit
GTACGGTCGCAAGATTAAAACTCAAATGAATTCTCG97090.16640592006757016No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGGAATTC95530.16373218193485403No Hit
TACGGTCGCAAGATTAAAACTCAAATGAATTGACGG95120.16302946870766583No Hit
TTCGAGGCAACGCGAAGAACCTTACCTACTCTTGAC94020.16114414053716083No Hit
TTCGATGCAACGCGAAGAACCTTACCTACTCTTGAC93050.15948162387771556No Hit
ACCCTGGTAGTCCACGCTGTAAACGATGTCTACTTG87580.1501064010662045No Hit
GAAGAACCTTACCTACTCTTGACATCCAGAGAATCT86690.14858099918279594No Hit
TGCTTGGCGACCATAGCGTTTTGGACCCACCTGAAT83800.14362772789846925No Hit
AGTACGGTCGCAAGATTAAAACTCAAATGAATTCTC82460.1413310553998541No Hit
TTAATTGAAGAGTTTGATCATGGCTCAGATTGAACG80750.13840022706206911No Hit
AAGCAACGCGAAGAACCTTACCTACTCTTGACATCC79160.13567507088833922No Hit
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGG77320.13252143103949454No Hit
TCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGG76610.1313045374021686No Hit
GAAGAACCTTACCTACTCTTGACATCCAGAGAAGCC75710.12976199617175546No Hit
AGGCTGAGACACGACGTCGAGCTGGAATTCTCGGGT75360.1291621190265948No Hit
ACGGTCGCAAGATTAAAACTCAAATGGAATTCTCGG74100.12700256130401638No Hit
CTCGTGTCGGTGGTTCGATTCCGCCTCCGGGCACCA74040.12689972522198883No Hit
CGGTCGCAAGATTAAAACTCAAATGAATTCTCGGGT73330.1256828315846629No Hit
AGTACGAGAGGACCGGAGTGGACGTGGAATTCTCGG68480.11737024828743645No Hit
TCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGT67710.11605051856808297No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGGA67530.11574201032200035No Hit
TAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTC63120.10818355829297589No Hit
TAACACATGCAAGTCGAGCGGCAGCACAGAGATGGA59970.10278466398652983No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)