FastQCFastQC Report
Tue 4 Jul 2023
SRR8258336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8258336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20039467
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG8073164.028630102786666No Hit
TAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG3542461.7677416270602406No Hit
TTGGAAACGATGGCTAATACCGCATGATGCCTACGGGCCAAAGAGGGGGA2276751.1361330119209259No Hit
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCA2197101.0963864458071664No Hit
GTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGG1663210.8299671842569465Illumina Small RNA Adapter 2 (100% over 21bp)
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGGAATTCTCGGGTGCC1398420.6978329313848517No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCAA1090280.5440663666354001No Hit
TTAGGTAAATCCGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1044350.5211465953660345RNA PCR Primer, Index 1 (100% over 30bp)
TCATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG1023390.5106872353441336No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGGAATTCTCGGGTGCCA949980.47405452450406993No Hit
ACTACGGTAGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAG889980.4441136084108424No Hit
GAGTAACATCAACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCA797060.3977451096877976RNA PCR Primer, Index 1 (100% over 28bp)
TTAGGTAAATCCGGACACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG709950.3542758896731136RNA PCR Primer, Index 1 (100% over 29bp)
CTACGGTAGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGC683530.3410919062867291No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGTATGGAATTCTCGGGTGCCAAGG575120.2869936610589493Illumina Small RNA Adapter 2 (100% over 21bp)
TTTAGGTAAATCCGGACACTCTTGGAATTCTCGGGTGCCAAGGAACTCCA520530.25975241756679457RNA PCR Primer, Index 1 (100% over 28bp)
AGTAACATCAACTGGAGGACCGATGGAATTCTCGGGTGCCAAGGAACTCC454920.22701202581885035RNA PCR Primer, Index 1 (100% over 27bp)
TTTAGGTAAATCCGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAG415010.20709632646417195RNA PCR Primer, Index 1 (100% over 29bp)
TTAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT399680.19944642240235233No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGTACTGGAATTCTCGGGTGCCAAG386290.1927646079608804No Hit
GAGTAACATCAACTGGAGGACCGTGGAATTCTCGGGTGCCAAGGAACTCC339640.16948554569839608RNA PCR Primer, Index 1 (100% over 27bp)
GTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCTCGGGTGCCAAG330620.16498442797904755No Hit
GAGTAACATCAACTGGAGGACCGATGGAATTCTCGGGTGCCAAGGAACTC321760.1605631527026143RNA PCR Primer, Index 1 (100% over 26bp)
TGAGACACGACGTCGAGCTACTTGGAATTCTCGGGTGCCAAGGAACTCCA276850.13815237700683355RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG274840.13714935631771044No Hit
GGGGCTGATTTAGGATTCGACGGGAATGGAATTCTCGGGTGCCAAGGAAC272410.13593674921593474RNA PCR Primer, Index 1 (100% over 24bp)
GCGTAACATCAACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCA267280.1333768008899638RNA PCR Primer, Index 1 (100% over 28bp)
AATAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG264650.13206439073454398No Hit
CATAAGCCAGTATCATTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC255280.12738861767131832RNA PCR Primer, Index 1 (100% over 31bp)
AGTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCTCGGGTGCCAA247050.12328172201386395No Hit
TTGTAAACGATGGCTAATACCGCATGATGCCTACGGGCCAAAGAGGGGGA236150.11784245559026095No Hit
GCCTTGCACGCAGGAGGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG214170.10687409999477532RNA PCR Primer, Index 1 (100% over 29bp)
AATACGGTAGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAG211000.10529222159451647No Hit
GGGGGTAGAGCACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG209230.10440896456976624RNA PCR Primer, Index 11 (100% over 35bp)
AGTAACATCAACTGGAGGACCGTGGAATTCTCGGGTGCCAAGGAACTCCA206190.10289195815437605RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGATTTAGGATTCGACGGGAATTGGAATTCTCGGGTGCCAAGGAA201460.1005316159356933RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG200510.10005755143088386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)