FastQCFastQC Report
Tue 4 Jul 2023
SRR8258337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8258337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26587915
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG298379611.22237678283536No Hit
GTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGG8542183.21280551709301Illumina Small RNA Adapter 2 (100% over 21bp)
TTTAGGTAAATCCGGACACTCTTGGAATTCTCGGGTGCCAAGGAACTCCA4741731.7834155103925977RNA PCR Primer, Index 1 (100% over 28bp)
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCA4611101.7342841663214283No Hit
AGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC2839261.0678761384636593RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAACT2793851.0507969504190156RNA PCR Primer, Index 1 (100% over 25bp)
GTGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGGAATTCTCGGGTGCC2603100.9790538295311987No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCAA2561950.9635768731771559No Hit
GTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCTCGGGTGCCAAG2424350.9118240373492995No Hit
TAAGGCTGAGACACGACGTCGAGCTACTTGGAATTCTCGGGTGCCAAGGA2267730.8529175755225635RNA PCR Primer, Index 1 (100% over 22bp)
TTAGGTAAATCCGGACACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2169660.8160323966734511RNA PCR Primer, Index 1 (100% over 29bp)
TTTAGGTAAATCCGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1773660.6670925493781668RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGTCGGGAACTCCAGGGAGACTGCCGTGGAATTCTCGGGTGCCAA1638540.6162724681495334No Hit
TGAGGAAGGTGGTGTCGTTAATAGCGGCATCATGGAATTCTCGGGTGCCA1542480.5801432718586621No Hit
AAGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAACTC1213230.456308815490045RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTGATTTAGGATTCGACGGGAATGGAATTCTCGGGTGCCAAGGAAC1179130.44348343975072885RNA PCR Primer, Index 1 (100% over 24bp)
TAAGGCTGAGACACGACGTCGAGCTATGGAATTCTCGGGTGCCAAGGAAC1114310.4191039425242633RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGACACGACGTCGAGCTATGGAATTCTCGGGTGCCAAGGAACTC1066090.40096788334098404RNA PCR Primer, Index 1 (100% over 26bp)
TTAGGTAAATCCGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1050510.39510807823780086RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG1007600.3789691670068902No Hit
AATAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG947600.3564025234773016No Hit
GGGGCTGATTTAGGATTCGACGGGAATTGGAATTCTCGGGTGCCAAGGAA897370.33751048173578113RNA PCR Primer, Index 1 (100% over 23bp)
TGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG856020.32195830323663965No Hit
GTCATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGG803570.30223129568452434Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAACT746420.28073656772259126RNA PCR Primer, Index 1 (100% over 25bp)
AGTAATGTCGGGACCTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG631910.23766812854637154No Hit
TTCGGACGGGGGTTCAAATCCCCCCAGCTCTGGAATTCTCGGGTGCCAAG603470.22697153951334656No Hit
TCAGGCTGAGACACGACGTCGAGCTACTTGGAATTCTCGGGTGCCAAGGA601310.22615914034628137RNA PCR Primer, Index 1 (100% over 22bp)
AGGCTGAGACACGACGTCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCA546390.20550313930219802RNA PCR Primer, Index 1 (100% over 28bp)
AGCAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG505680.1901916716673722No Hit
GTTAGGTAAATCCGGACACTCTTGGAATTCTCGGGTGCCAAGGAACTCCA475200.1787278167543412RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGATTTAGGATTCGACGGGATGGAATTCTCGGGTGCCAAGGAACT469080.17642601911432318RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTGATTTAGGATTCGACGGGAATTTGGAATTCTCGGGTGCCAAGGA458860.17258216749978325RNA PCR Primer, Index 1 (100% over 22bp)
AAGGCTGAGACACGACGTCGAGCTATGGAATTCTCGGGTGCCAAGGAACT433850.16317563825519976RNA PCR Primer, Index 1 (100% over 25bp)
AAGGCTGAGACACGACGTCGAGCTGGAATTCTCGGGTGCCAAGGAACTCC429010.16135526234381298RNA PCR Primer, Index 1 (100% over 27bp)
TTTAGGTAAATCCGGACACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT423140.15914749238516823RNA PCR Primer, Index 1 (100% over 30bp)
GCAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGG412620.15519080755298037Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGCTGAGACACGACGTCGAGCTACTTGGAATTCTCGGGTGCCAAGGAA402430.15135823926020525RNA PCR Primer, Index 1 (100% over 23bp)
GAAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGG400360.15057969005843444Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGGCTGAGACACGACGTCGAGCTGGAATTCTCGGGTGCCAAGGAACTC399070.15009450722254827RNA PCR Primer, Index 1 (100% over 26bp)
CTTAAGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAA394110.1482289980241023RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCTGAGACACGACGTCGAGCTTGGAATTCTCGGGTGCCAAGGAACTC386960.14553980633682634RNA PCR Primer, Index 1 (100% over 26bp)
AGAAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG374130.14071430572874932No Hit
AGTGATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG369490.13896915196246112No Hit
AATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAGGAA353180.132834786029668RNA PCR Primer, Index 1 (100% over 23bp)
TCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC345790.13005532776827367RNA PCR Primer, Index 1 (100% over 33bp)
GGGTCTGTAGCTCAGGTGGTTAGAGCGTATGGAATTCTCGGGTGCCAAGG336450.12654245359216773Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGCCAGTATCATTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC326760.12289794066213917RNA PCR Primer, Index 1 (100% over 31bp)
AGGCTGAGACACGACGTCGAGCTACTTGGAATTCTCGGGTGCCAAGGAAC320190.12042689319564923RNA PCR Primer, Index 1 (100% over 24bp)
TTCGGACGGGGGTTCAAATCCCCCCAGCTCCTGGAATTCTCGGGTGCCAA318270.11970476060270238No Hit
AGTAATTTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCGGGTGCCAAG300110.11287458982774694No Hit
TAAGGCTGAGACACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC296210.11140775799832368RNA PCR Primer, Index 1 (100% over 33bp)
TCAGGCTGAGACACGACGTCGAGCTATGGAATTCTCGGGTGCCAAGGAAC295730.11122722485008697RNA PCR Primer, Index 1 (100% over 24bp)
GAGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCA288580.10853803316281099No Hit
AACTGGAGGACCGAACCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG285050.10721036230182021RNA PCR Primer, Index 1 (100% over 29bp)
GCGAGGAAGGTGGTGTCGTTAATAGCGGCATCTGGAATTCTCGGGTGCCA283310.10655592963946213No Hit
AGTAATGTCGGGAACTCCAGGGAGACTGCCTGGAATTCTCTGGTGCCAAG282560.1062738465953423No Hit
GTTAATAGCGGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT280940.10566454722004338RNA PCR Primer, Index 3 (100% over 35bp)
GAGTAACATCAACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCA280130.10535989753239394RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)