FastQCFastQC Report
Mon 10 Jul 2023
SRR8295887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8295887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285668
Total Bases8.5 Mbp
Sequences flagged as poor quality0
Sequence length15-43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT96313.3713961661789207No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCC55681.9491157567525939No Hit
CTTTTTTCGAAGCATTAAGCGGAGCC40621.42193035271714No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCC21530.7536720948793705No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTC21530.7536720948793705No Hit
CTAAATCACTTGCATACGACTTGGTCT20430.7151658568688127No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGT15190.5317361412548833No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAG13950.4883291093157091No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT11320.39626419479955755No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCG9590.33570438411022585No Hit
GACTATCCTTTGATTGAGTTTTTTGT9310.32590279625299295No Hit
AGAGAAGGTGTCTTGCTGGCGGT8780.30734979066608786No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAG8220.2877466149516221No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTT8090.2831958777321926No Hit
AGACTATCCTTTGATTGAGTTTTTTGT7540.26394275872691375No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGT6890.24118907262976602No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGC6820.2387386756654578No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCC6740.23593822199196268No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCC6710.23488805186440204No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCC6240.21843538653261826No Hit
GGGAATAGGGTTAATATTCCCTAACTGAGATGC5990.20968396880294607No Hit
AGGGAATAGGGTTAATATTCCCTAACTGAGATGC5970.2089838553845723No Hit
AAGGTAGCTGTAGGTGAACCTGCAGCTGGATCATCG5610.19638181385384432No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAT5400.18903062296091966No Hit
CTAAATCACTTGCATACGACTTGGTC5160.18062926194043435No Hit
AGAGAAGGTGTCTTGCTGGCGGC4500.15752551913409973No Hit
CTAAATCACTTGCATACGACTTGGTCC4420.15472506546060463No Hit
GTGGGTTCGTGGCGGCTAGCGTTT4220.14772393127686687No Hit
CACCCACGCGGCCCGGGTTCGATTCCCGGCATGGGAACC4030.141072853802316No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTC4020.1407227970931291No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCT4000.14002268367475532No Hit
TACCTGATTGATTCTGTCAGCGCGATATGCTC3990.13967262696556842No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGT3940.137922343419634No Hit
AGAGAAGGTGTCTTGCTGGCGG3870.1354719464553258No Hit
GGAATAGGGTTAATATTCCCTAACTGAGATGC3700.12952098239914867No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCC3530.12357001834297156No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGC3410.1193693378327289No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCC3370.11796911099598134No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCT3330.11656888415923379No Hit
TCTATTAATTAATCTGACGTGATATTAG3170.11096797681224357No Hit
GGTTCCATGGTGTAGCGGTTAGCACTCAGGACT3050.10676729630200092No Hit
TAGGTGGGAGTCTTCGGACGACAGTGAAATACCACCACT3040.10641723959281403No Hit
TTTTTTCGAAGCATTAAGCGGAGCC3040.10641723959281403No Hit
GAATAGGGTTAATATTCCCTAACTGAGATGC2980.10431689933769271No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCC2980.10431689933769271No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT2900.1015164456641976No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTC2890.1011663889550107No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGC2890.1011663889550107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)