FastQCFastQC Report
Mon 10 Jul 2023
SRR8295891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8295891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331913
Total Bases9.1 Mbp
Sequences flagged as poor quality0
Sequence length15-42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAATCACTTGCATACGACTTGGTCT153384.6210904664776615No Hit
CTAAATCACTTGCATACGACTTGGTC96052.8938306122387494No Hit
CTAAATCACTTGCATACGACTTGGTCC37371.1258974490303182No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAG27920.8411842862436844No Hit
CTAAATCACTTGCATACGACTTGGCC22640.6821064556073428No Hit
TAAATCACTTGCATACGACTTGGTCT20440.6158240261755339No Hit
CACCCACGCGGCCCGGGTTCGATTCCCGGCAT18520.5579775423077734No Hit
CACATGCGAGACCCGGGTTCATTTCCCGGCCG17660.532067138075339No Hit
CACCCATAAGGCCGGGGTTCGATTCCCCGC16870.5082657202339167No Hit
GACTATCCTTTGATTGAGTTTTTTGT15170.4570474793093371No Hit
TAAATCACTTGCATACGACTTGGTC13710.4130600488682275No Hit
CATGCGAGACCCGGGTTCATTTCCCGGCCG12860.3874509284059377No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGT12700.3826303880836243No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT11770.3546109974601778No Hit
CATGGGAGAGGGCTGGGGTTCAATTCCCCATAC11490.3461750518961294No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTG11270.3395468089529485No Hit
CAATGAGTTGAATACAAATGATTCTTCG10970.33050829584861097No Hit
GTCACATGCGAGACCCGGGTTCATTTCCCGGCCG10850.3268928906068759No Hit
GCATGGGAGAGGGCTGGGGTTCAATTCCCCATAC10290.3100209994787791No Hit
TACTGCGATCTGTTGAGACTATCCTTTG10140.3055017429266103No Hit
ACCCATAAGGCCGGGGTTCGATTCCCCGC10120.3048991753863211No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCT9580.28862985179851347No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCG9000.2711553931301275No Hit
CAACACGAGAGCTTATGCCCT8740.2633220151063682No Hit
ATGGGAGAGGGCTGGGGTTCAATTCCCCATAC8650.260610461175067No Hit
CTAAATCACTTGCATACGACTTGGTCG8650.260610461175067No Hit
GCTAAATCACTTGCATACGACTTGGTCT8420.2536809344617415No Hit
TCGGGAGGCATCTCTATCTCGTG8220.24765525905884977No Hit
ACATGCGAGACCCGGGTTCATTTCCCGGCCG8090.24373857004697017No Hit
CTTGGTCAAGGTGTTGTATTCAGT7930.23891802972465676No Hit
CAAGCATTCGACGGGGGTTCGATTCCCCCCG7710.23228978678147588No Hit
CAACACGAGAGCTTATGCCCTAT7110.2142127605728007No Hit
AGACTATCCTTTGATTGAGTTTTTTGT7050.2124050579519332No Hit
ATGGGAGAGGGCTGGGGTTCAATTCCCCATACCTCC6750.20336654484759562No Hit
CACCCACGCGGCCCGGGTTCGATTCCCGGCATGGG6590.19854600452528223No Hit
CCATAAGGCCGGGGTTCGATTCCCCGC6560.1976421532148485No Hit
AACACGAGAGCTTATGCCCT6530.1967383019044147No Hit
CCGTGGCGACGCTGGTTCGTTTCCAGCCGC6260.18860364011051087No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT6110.1840843835583421No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCC6030.1816741133971854No Hit
TACTGCGATCTGTTGAGACTATCCTTT5920.17835999192559496No Hit
ACCCACGCGGCCCGGGTTCGATTCCCGGCAT5870.17685357307487204No Hit
TAAATCACTTGCATACGACTTGGTCC5740.17293688406299243No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGC5710.17203303275255866No Hit
TCGGGAGGCATCTCTATCTCGTGG5680.17112918144212488No Hit
TGCGAGACCCGGGTTCATTTCCCGGCCG5670.1708278976719803No Hit
TGCGAGAGGTCCCGGGTTCAGTCCCCGGTTC5590.16841762751082362No Hit
TATGATTTTTGGAACCTAGGG5290.15937911440648603No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGT5280.15907783063634146No Hit
GCATGGGAGAGGGCTGGGGTTCAATTCCCCATACCTCC5250.15817397932590768No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGC5180.1560649929348956No Hit
CACGCGGGCGGCCCGGGTTCGTTTCCCGGTCG5170.15576370916475102No Hit
ATGCGAGACCCGGGTTCATTTCCCGGCCG5150.15516114162446182No Hit
CTCACCCATAAGGCCGGGGTTCGATTCCCCGC5110.15395600654388347No Hit
CAAGGAGAGGGCAAGTCTGGT5070.15275087146330515No Hit
GCAATTTTTGAACGCCTCTAGGAGGCAG5010.1509431688424376No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTT4980.15003931753200386No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTG4950.14913546622157012No Hit
CATGCGAGAAGTCTGGGGTTCGATTCCCCATAC4950.14913546622157012No Hit
ACTGCGATCTGTTGAGACTATCCTTTG4890.1473277636007026No Hit
CATGGGAGAGGGCTGGGGTTCAATTCCCCATACCTCC4880.147026479830558No Hit
CAATGAGTTGAATACAAATGATTCTTC4770.14371235835896756No Hit
AAGCATTCGACGGGGGTTCGATTCCCCCCG4670.1406995206575217No Hit
TCCTCGGTAGTATAGTGGTGAGTATCC4660.14039823688737713No Hit
ACGCAATTTTTGAACGCCTCTAGGAGGCAG4600.1385905342665096No Hit
AACACGAGAGCTTATGCCCTAT4590.138289250496365No Hit
TAAATCACTTGCATACGACTTGGCC4560.13738539918593126No Hit
TGCGAGAGGTCCCGGGTTCATTCCCCGGTTC4530.1364815478754975No Hit
TCACCCATAAGGCCGGGGTTCGATTCCCCGC4410.13286614263376245No Hit
GACTATCCTTTGATTGAGTTTTTTGC4350.13105844001289496No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGG4330.13045587247260576No Hit
ATGCGAGAAGTCTGGGGTTCGATTCCCCATAC4300.12955202116217202No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGG4220.12714175100101532No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGG4190.12623789969058158No Hit
CACCCATAAGGCCGGGGTTCGATTCCCCGCAAC4110.12382762952942489No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTG4050.12201992690855737No Hit
GTCGGGAGGCATCTCTATCTCGTG3990.12021222428768984No Hit
GGAGGCATCTCTATCTCGTG3850.11599425150566564No Hit
TCATATGGATAACTGCGGAAATACTGGAGCTAATACATGC3790.11418654888479814No Hit
CGCAATTTTTGAACGCCTCTAGGAGGCAG3720.11207756249378602No Hit
CACAGAAGGTCGGTGGTTCGAGCCCGCCCG3630.10936600856248475No Hit
AATGAGTTGAATACAAATGATTCTTCG3580.10785958971176182No Hit
ACAGAAGGTCGGTGGTTCGAGCCCGCCCG3570.10755830594161722No Hit
TGTGGTCGTGACTTCTAATTTGCTGGT3560.10725702217147264No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCC3370.10153263053872552No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGC3360.10123134676858092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)