FastQCFastQC Report
Fri 7 Jul 2023
SRR8302260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48518526
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC17381263.582396546836563No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC13117792.70366622432017No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT9909852.0424878529904227No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC8639671.7806950689309893No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA8470141.7457537766089597No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC7242891.492809159124084No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG7050681.4531933637060614No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6745931.3903823046891408No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA6222551.282510107582411No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT5617901.1578876077150406No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG5548401.143563182442929No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC5033351.037407855300468No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT4992201.0289265588983474No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG4500160.9275137501085667No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG3967730.8177762861138856No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA3883120.8003375865128303RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC3759120.7747803385453218No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT3757410.7744278958515763No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC3704390.7635001112770821No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG3700430.7626839281968294No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC3504280.7222560718353233No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA3341300.6886647793051257RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3125530.6441931067732767No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2790140.57506693422632Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2194630.4523282508623613Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA2033340.4190852788891402RNA PCR Primer, Index 1 (100% over 23bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC1930970.3979861218372545No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1864900.3843686430210184No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1836050.37842246073180374No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1815250.3741354384920927RNA PCR Primer, Index 1 (100% over 24bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1708630.3521603273768045No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1669200.34403353473681375No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1667950.3437759011887542No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1662850.34272475631267113RNA PCR Primer, Index 1 (100% over 23bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTC1543600.3181465158277892No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1530800.31550834829565927No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA1508990.31101315814911606RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1495310.30819361659915223No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC1401360.2888298791269957RNA PCR Primer, Index 10 (100% over 50bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1335340.27522270565268203No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC1301480.26824392810284464RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG1283010.26443713479671666No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1282240.26427843253111194No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA1226410.2527714877405798RNA PCR Primer, Index 1 (100% over 22bp)
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGTGGAATTCTCGGGTGCCA1209710.24932950353850403No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTC1185660.24437263407383808No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG1168280.24079049722161797Illumina Small RNA Adapter 2 (100% over 21bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1167690.24066889418693388No Hit
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA1150570.23714034511271015RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1144910.23597378040709646Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1126090.23209484970751174RNA PCR Primer, Index 1 (100% over 24bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCT1120690.23098187277989443No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG1084140.2234486678346329No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA1081370.2228777518921329RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC1057330.21792294349585145No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1050110.21643485212225946No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1034010.21311653202325231RNA PCR Primer, Index 1 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC1021600.21055874615811698No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1019760.21017950957537332No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1011270.2084296625169528RNA PCR Primer, Index 1 (100% over 32bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGG1001180.20635004451701605No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG991210.204295159337693No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC990080.20406225861024715No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG980480.20208363296114973No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG942550.19426600057883045Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA936890.19309943587321676No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC916060.18880623042835223RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT860980.1774538657666558No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG856480.1765263849936414No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC855190.17626050717204392No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG847210.1746157746012317No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA827380.17052867599481486No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA819230.16884890526146654No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCG804590.16583150114659295No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA778420.160437685184418RNA PCR Primer, Index 1 (100% over 22bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA775330.15980081505361476RNA PCR Primer, Index 1 (100% over 32bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT753880.15537982336891273No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT746480.15385463276440015RNA PCR Primer, Index 1 (100% over 25bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG746380.15383402208055538No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG745960.15374745720840735Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA735500.15159157967824496RNA PCR Primer, Index 1 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA732710.15101654159897604No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA729810.15041883176747783RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC715330.1474344047467559No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC714540.14727158034438226RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG704420.1451857791392921No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC693380.14291035964283003No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG691730.14257028335939143No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA681180.14039585621376874No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA669130.13791226881047458No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTG657730.1355626508521714No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA653440.13467845251523097No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT649440.1338540251614404RNA PCR Primer, Index 1 (100% over 25bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG634960.13086959814071847No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC618910.12756158338363371No Hit
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG617060.12718028573250556Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA614790.1267124232092294No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA614560.12666501863638643RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC614320.126615552995159No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA596810.1230066222539407No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC596530.12294891233917535RNA PCR Primer, Index 1 (100% over 31bp)
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGCC593860.12239860708052014No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA586080.12079509587739742RNA PCR Primer, Index 1 (100% over 28bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTG581180.11978517236900395No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG579460.119430668606874No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCG559400.11529616542761419No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA558330.1150756311104752No Hit
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC552710.11391731067839943RNA PCR Primer, Index 10 (100% over 44bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG547920.11293005892223519No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC545010.11233028802235254No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG537960.11087723481129662No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATT535660.11040318908286703No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC528360.1088986091621992RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA527550.1087316626230566RNA PCR Primer, Index 1 (100% over 32bp)
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA510420.10520105248044839No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA509090.10492693038531302RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG508380.10478059453001519Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGG506440.10438074726342675No Hit
GCAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAA505820.10425296102358922No Hit
TAGGATGGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAG503060.10368410614947372No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA501280.1033172359770369RNA PCR Primer, Index 1 (100% over 22bp)
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTGGAATTCTCGGGTGCC494770.10197548045874272No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTCTC488440.1006708241713691No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCA487460.1004688394696904No Hit
TGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGG485340.10003189297218139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)