FastQCFastQC Report
Tue 4 Jul 2023
SRR8302262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49824030
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT22045334.424638071227879No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC17826663.577924146240278No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG14851182.98072636838088No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC10866642.1810038248612167No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA8756611.7575073714430567RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC5999091.204055553113628RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA5636701.1313215731445248RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC5137461.0311209269904502RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4757100.9547802536246064No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT4250400.8530823379802879No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC4036610.8101733239964732No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3821480.7669953634822394Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC3797420.7621663683166536No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG3738140.7502684949410957RNA PCR Primer, Index 1 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3310690.6644765588010444No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA3133210.628855192966125No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG3026180.6073735906148098No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG3003740.6028697397621188No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA2898570.5817614512515347RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2671990.536285402846779RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG2645320.5309325640659738No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA2615680.5249836273781948RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC2455400.4928144110382079No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA2388070.4793008514164751No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG2320850.4658093694950007Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA2306810.4629914521165791RNA PCR Primer, Index 1 (100% over 23bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1973520.3960980273976232No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1967620.394913859838315No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1906440.3826346443673866No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGGA1860360.3733860950228233RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1851090.3715255470101475RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1784560.35817255248120233RNA PCR Primer, Index 1 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1776930.35664116290874104No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1662780.33373053123161656RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1617090.3245602573697872No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1616180.32437761457674136No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1590200.31916326318846544RNA PCR Primer, Index 2 (100% over 50bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1570260.31516117825073564RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1526070.306291963937883No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC1520010.3050756833600172No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1479510.2969470755376472Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1476440.29633090699407494No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT1432510.28751387633637826RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1402060.2814023674921519RNA PCR Primer, Index 1 (100% over 23bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC1394220.27982882958283384No Hit
TGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA1352780.2715115577764384RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC1309060.2627366754555984RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGG1289020.25871451988127014Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1225430.24595160206831923No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCTGGAATTCTCGGG1140300.22886546913206338No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1124690.2257324427590462No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1121130.22501792809614157No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1119060.22460246591855373No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG1108120.2224067382746839No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1056010.21194792954323444Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1043380.20941300814085093No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA1023090.2053406759750265RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAA1000900.20088700171383167RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG984410.19757735373874813No Hit
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA968490.19438210839227577RNA PCR Primer, Index 1 (100% over 32bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG941800.18902525548415092No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA940300.18872419593517425RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT930490.18675526648486684RNA PCR Primer, Index 1 (100% over 25bp)
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC893490.17932913094344236RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT893490.17932913094344236RNA PCR Primer, Index 1 (100% over 25bp)
AACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT889900.17860859508955818RNA PCR Primer, Index 1 (100% over 30bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG878160.1762523023529008Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC864920.17359495006726675No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC826460.16587578323150495RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC812520.16307793648968177No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA805760.16172116145562693No Hit
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA799910.16054702921461794RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC794980.15955754683031462No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT791720.15890324407720532No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC775130.15557352546552336No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG754610.15145503083552253No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC754170.1513667200344894No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA751470.15082481284633137RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC746610.14984937990764696RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG746000.14972694902439646No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC737720.14806510031404524RNA PCR Primer, Index 1 (100% over 31bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG737640.1480490438047665No Hit
AGCAGCCGACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG731270.14677054425344557RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG721100.14472936051138377No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA713130.14312973077448773RNA PCR Primer, Index 1 (100% over 22bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA707160.1419315137695606RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTGGAATTCTCGGGTGCCAAGGAACT691220.13873225429576852RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC690890.13866602119499366RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG685850.13765446111043206No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG677060.13589025215342876No Hit
CGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC669770.13442710274540218RNA PCR Primer, Index 1 (100% over 33bp)
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT656660.1317958422873461RNA PCR Primer, Index 1 (100% over 25bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA647640.12998547086616638RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC640610.12857450511329574RNA PCR Primer, Index 1 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAA639360.12832362215581516RNA PCR Primer, Index 1 (100% over 23bp)
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGTGGAATTCTCGGGTGCCA634810.12741040819058597No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA629850.1264149046153031RNA PCR Primer, Index 1 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC621410.12472094288639438RNA PCR Primer, Index 1 (100% over 26bp)
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC574480.11530179313074433No Hit
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC573580.11512115740135835RNA PCR Primer, Index 1 (100% over 27bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA562550.1129073661845499No Hit
CGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAA550810.1105510734478925No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG546400.10966595837390111No Hit
GGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACT531970.10676976551274556RNA PCR Primer, Index 1 (100% over 25bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC527290.10583045971993835RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA524120.10519422053976767No Hit
CAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAAG520510.10446967055856379No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA505340.10142495498657977No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG504610.10127843933941112No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACTGGAATTCTCGGGTG504040.10116403671079999No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG499530.10025885100021013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)