FastQCFastQC Report
Tue 4 Jul 2023
SRR8302263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49364568
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC13303682.694985601818697No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT13288592.691928753433029No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC13082082.650095104650769No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG9797281.9846785654034287No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC9542511.9330686738715102RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5833601.1817382864567965No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5258591.065255954432742No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA4962001.0051743995814975RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA4729400.9580555835108291RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT4572440.9262594985131847No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC4330730.877295229242156RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3953460.800869968111541Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG3315790.6716943213196963No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC3213300.6509324663795295No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2915840.5906746717605226No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG2710050.5489868765791691No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC2595970.525877183813297No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2439220.4941236394492503No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG2360850.47824788013945546No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG2241640.4540989804671237No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA2195560.44476435000909964No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC2155770.43670391281455156No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2124030.43027419990791776No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2113880.4282180692840257No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1904960.38589621608761976No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1904770.3858577269429361RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCCGACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1891530.3831756412818198RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1876860.3802038741633473RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1860380.3768654472981512Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1790360.3626811846099818RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1754730.35546345710956084Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1752360.3549833556732432No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1748330.3541669806570575No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1725210.3494834594723892No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA1689560.34226168048305416RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG1596160.3233412272543335Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1573160.31868201500314963RNA PCR Primer, Index 1 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1551350.31426386634235304No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1536260.31120701795668504No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT1491740.30218840363395866RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1464740.2967188935999602RNA PCR Primer, Index 1 (100% over 24bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1459540.2956655064823012No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1432410.2901696617703613No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAA1431460.2899772160469428RNA PCR Primer, Index 1 (100% over 23bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG1405720.28476294981453093No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA1398250.2832497187051247RNA PCR Primer, Index 1 (100% over 23bp)
AACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1274700.2582216459384391RNA PCR Primer, Index 1 (100% over 30bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1221780.24750140627180206Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1217640.24666274806658897No Hit
TGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA1207350.24457825702029842RNA PCR Primer, Index 1 (100% over 28bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGG1203610.24382062859336678Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCTGGAATTCTCGGGTGCCAAGGAACTCC1191990.24146671353429042RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGGA1177230.23847671471570459RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC1162830.23555964269757207No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1131870.22928793785858717No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1073990.2175629289412601No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1073190.21740086938469716RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1069820.21671819350267585RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT1062130.2151603960152148No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1061950.21512393261498816RNA PCR Primer, Index 1 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1029020.20845315611796705No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA1020550.20673735056285716RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA996980.20196267087762218RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA987540.20005036811017976No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT968310.1961548615192986No Hit
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC959530.1943762578860206RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA947270.19189269518169388No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA945430.19151995820159917No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCTGGAATTCTCGGG940770.19057596128462018No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA909250.1841908147560412RNA PCR Primer, Index 1 (100% over 28bp)
GGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACT909040.18414827412244344RNA PCR Primer, Index 1 (100% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA906080.18354865376316065No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA896220.1815512697285227RNA PCR Primer, Index 1 (100% over 23bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC877180.17769425228232524No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA874950.17724251126840612RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC836460.16944542085327274RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC791860.1604106005748901RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG790570.16014927953993235No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG789010.15983326340463466No Hit
AGCAGCCGACTTAGAACTGGTGTGGAATTCTCGGGTGCCAAGGAACTCCA783840.15878595352034683RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT777780.1575583523793827RNA PCR Primer, Index 1 (100% over 25bp)
CGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAA777720.1575461979126405No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG767670.15551032473331883No Hit
ACGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT757750.15350078623193866RNA PCR Primer, Index 1 (100% over 30bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA751990.15233395742468564RNA PCR Primer, Index 1 (100% over 32bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA719740.14580093155074303RNA PCR Primer, Index 1 (100% over 22bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA714990.14483870293365073No Hit
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA708570.1435381749922333RNA PCR Primer, Index 1 (100% over 22bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC693920.14057045936267487No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC689670.13970951796843437No Hit
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGC687760.1393226007771404No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGTG676190.1369788144403492No Hit
CGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC671650.13605912645685464RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA671620.13605304922348355No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGATGGAATTCTCGGGTGCCAAGGAA669570.13563777160979104RNA PCR Primer, Index 1 (100% over 23bp)
ACGAGAACTTTGAAGGCCGAAGTGGAGATGGAATTCTCGGGTGCCAAGGA664250.13456007555864766RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA663720.1344527111024247No Hit
ACGAGAACTTTGAAGGCCGAATGGAATTCTCGGGTGCCAAGGAACTCCAG656970.1330853335939251RNA PCR Primer, Index 1 (100% over 29bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGGTGC635740.12878467811163669No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA634080.12844840453176862RNA PCR Primer, Index 1 (100% over 23bp)
ACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA633330.12829647369749086RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA629770.1275753086707859RNA PCR Primer, Index 1 (100% over 22bp)
CGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG629550.1275307422927311RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA629040.12742742932542223RNA PCR Primer, Index 1 (100% over 28bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG625450.12670018706534614No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT624700.12654825623106838RNA PCR Primer, Index 1 (100% over 25bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA610490.12366967335761957No Hit
CGGCGTCCGGTGAGCTCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA597270.12099163918541736RNA PCR Primer, Index 1 (100% over 28bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA591120.11974580634433994RNA PCR Primer, Index 1 (100% over 22bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG591080.1197377033665118Illumina Small RNA Adapter 2 (100% over 21bp)
CAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAAG590850.11969111124399995No Hit
GAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG570750.11561936488535664RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC570320.11553225787370408RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA568280.11511900600446863No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC552070.11183527423961251No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC549920.11139973918134966No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC549150.11124375685815785RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG546070.11061982756539063No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC539860.10936184025757097RNA PCR Primer, Index 1 (100% over 31bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC534240.10822337187271647No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA523940.10613685508196892RNA PCR Primer, Index 1 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAA512380.10379509448963475RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG508190.10294630756213646No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT508040.10291592139528093RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA505580.10241758825884995No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC505170.10233453273611146No Hit
GCAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAA503420.10198002745613008No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA501890.10167008855420348RNA PCR Primer, Index 1 (100% over 34bp)
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGTGGAATTCTCGGGTGCCA499490.10118390988451474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)