FastQCFastQC Report
Tue 4 Jul 2023
SRR8302265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50454871
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC16403163.2510557801247773No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT13817412.7385680958336014No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC11452782.2699057143561028No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG10048411.9915639066840543No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA6323851.253367588631829RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC6313041.2512250799333131RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6241521.2370500362591355No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA6031721.1954683225728593RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG4947730.9806248439323133Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT4659730.9235441311503898No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA4220110.8364128014518163No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC4041110.8009355528824957No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA3935180.7799405532123945RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC3760020.7452243808134996RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3708310.7349756181122731No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA3510520.6957742494277709No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG3239410.6420410826142039No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2930580.58083192800156RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2840170.5629129445202625No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2827580.5604176453052471RNA PCR Primer, Index 3 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2741440.5433449626697093No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG2722160.5395237260640307No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA2697900.534715468799831RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2645140.5242585993332537RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2582350.5118138147652781No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2569170.5092015793678275No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC2513520.49817192080423717No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG2433190.4822507622703069No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC2312170.4582649710867361No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA2269600.4498277282286581RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2034780.40328712761945223No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1947450.38597859064985024No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1873310.3712842710468926Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1788070.35438996563879827No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1764300.34967882486509577No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA1758440.34851739091751915RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1731100.34309868714162406Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG1682550.33347622670564353Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1681660.33329983144739384No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT1584720.3140866220825339RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1554790.3081545882854403RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC1535930.3044165943859018RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1437590.28492590933390755No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1429020.2832273617348065RNA PCR Primer, Index 3 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1330130.26362766837715235RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGGA1314200.26047039145140216RNA PCR Primer, Index 1 (100% over 22bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1297980.25725563741903135No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1291830.256036726364834No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1247690.24728831434332674RNA PCR Primer, Index 1 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA1247600.24727047662058238RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1188720.23560064200738914No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1187150.2352894728439599RNA PCR Primer, Index 1 (100% over 23bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC1185110.2348851511284213No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT1184600.23478407069953663RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1153730.22866573179822422No Hit
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1139600.2258652093273611RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1130390.22403981569985582No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGG1115600.22110848326220076Illumina Small RNA Adapter 2 (100% over 21bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1091040.21624076692218674RNA PCR Primer, Index 3 (100% over 44bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1083890.21482365894860775No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA1066540.2113849423973356RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG1047080.20752803034616818No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA986260.19547369370937445RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC962120.19068922007550074RNA PCR Primer, Index 1 (100% over 24bp)
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA944610.18721879201712754RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA943050.18690960482289212RNA PCR Primer, Index 1 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAA931530.1846263763116152RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC906890.17974280421805064RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC899520.17828209292220765No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG883300.17506733888983683No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT872550.17293672200648377No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA848310.16813242868067188No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC820280.16257696903040342RNA PCR Primer, Index 1 (100% over 24bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG806360.15981806791261047Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA802940.15914023444832512No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTGGAATTCTCGGGTGCCAAGGAACT764000.15142244640760255RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCTGGAATTCTCGGG759380.1505067766400592No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC756020.14984083499093676RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA755510.1497397545620521No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC733020.14528230584515814RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC716780.14206358787439968RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG709420.14060485854775054No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT708070.14033729270658526RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA694150.1375783915887923No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG687380.13623659844457833No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC685400.1358441685442026RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC683060.13538038775284947No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC679350.13464507718194343No Hit
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA672840.13335481523676873RNA PCR Primer, Index 1 (100% over 32bp)
TGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA670450.1328811245994465RNA PCR Primer, Index 1 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG659490.1307088863630233Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC653040.12943051623301147No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG645790.12799358856749432RNA PCR Primer, Index 3 (100% over 45bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG643340.1275080061150092No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC629540.12477288862754203RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA623220.12352028409704982RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG618410.12256695691482394No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAA604270.11976445247476701RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG597370.11839689373103343Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA591760.11728500901330219RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA589230.11678357080726655No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA574160.11379674323218467RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG569740.11292071284851764Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC568530.11268089457606581No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT567220.11242125661167582RNA PCR Primer, Index 1 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGA553950.10979118349148094RNA PCR Primer, Index 1 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA545170.10805101453930979No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG536320.10629697180278193No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG535200.10607499125307444Illumina Small RNA Adapter 2 (100% over 21bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT534450.1059263435635382No Hit
AGCAGCCGACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG533850.10580742541190918RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG532290.10549823821767376No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT527720.10459247829609951RNA PCR Primer, Index 3 (100% over 46bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC525510.10415446310426599No Hit
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC521130.10328636059737423RNA PCR Primer, Index 1 (100% over 26bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG517420.1025510500264682No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC516550.10237861870660615RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTATGGAATTCTCGGGTGCCAAGGAACTC514670.10200600849816858RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA514130.1018989821617025RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAAG509320.1009456549794766No Hit
AACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT508890.10086043030414248RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)