FastQCFastQC Report
Tue 4 Jul 2023
SRR8302267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49972480
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC16607143.323257120719244No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT14329312.867440239107605No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC10644852.1301424303936884No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG10045092.010124372454599No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA7999071.6006950225404062RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC7611491.5231363342383648RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC7433611.4875407424246305RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA6926631.3860889033323942RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6246121.2499119515381267No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA4514730.9034432551676442No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4136080.8276715504213519No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3636420.7276845175584642No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3560800.7125521887246741Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA3461080.6925972055019083No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA3208060.6419653377218821RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2889870.5782922920775595RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2867240.5737637995952972No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2843490.569011183755539No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA2636680.5276264055736277RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG2568240.5139308675494992Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA2537440.5077674752183602RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2533360.5069510258446249No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGGA2511280.5025325939397044RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2494650.4992047623011706RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG2400440.4803523859532286No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA2328640.4659844778566123RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2233680.44698201890320427No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2231810.44660781294024227Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC2052400.4107060526113573RNA PCR Primer, Index 5 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2031600.40654376168643225No Hit
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1977830.39578383942521966RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1923550.38492186099229014No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1871870.3745801689249763Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1869460.37409790348607874No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1831770.36655575228605825RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1679430.33607097346379444RNA PCR Primer, Index 1 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1673810.33494635447350224No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1655970.3313763895648165No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1625760.32533106221664404No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1523520.3048718014395123No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC1509350.3020362407469071RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1447120.2895833866960375No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC1425750.2853070329909582RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCTGGAATTCTCGGG1408930.2819411804257063No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1408520.28185913526805156No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT1348020.26975247176045697RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1343150.26877793537563077No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGG1338910.267929468379396Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1337150.26757727453190233Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1269560.2540518301273021RNA PCR Primer, Index 1 (100% over 23bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1250460.2502297264414334RNA PCR Primer, Index 5 (100% over 44bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC1239240.24798449066366127No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG1191340.2383992149278963No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT1179230.2359758811249712RNA PCR Primer, Index 1 (100% over 25bp)
AACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1164020.2329322058861197RNA PCR Primer, Index 1 (100% over 30bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1149400.2300065956302349No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1143400.22880593478650646RNA PCR Primer, Index 1 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1137160.22755724750902895No Hit
TGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA1132160.22655669680592197RNA PCR Primer, Index 1 (100% over 28bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAA1127710.22566620668015677RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1097980.2197169321994826No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA1058150.21174654529853232RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA1025520.2052169514100561RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA991380.19838519120924156RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA959110.19192763697138904RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA958660.19183758740810944RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT958100.19172552572936144RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT950810.19026672280423146RNA PCR Primer, Index 5 (100% over 43bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG891290.17835616723444583No Hit
CGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC854150.17092407661176712RNA PCR Primer, Index 1 (100% over 33bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC843320.16875688378883735No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA829210.16593332970466945RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGCCGACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG825120.16511487922952794RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC803310.16075047706257525RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA802590.16060639776132785No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC792270.15854126111011502No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA788050.15769679631669273No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACTGGAATTCTCGGGTG778300.1557457224456341No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC776940.15547357265438896RNA PCR Primer, Index 1 (100% over 24bp)
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA774560.15499731051971005RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC769670.1540187719320714RNA PCR Primer, Index 1 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAA719180.14391521093209703RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG711570.14239237276196817No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG709130.14190410401885198No Hit
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG684230.13692136151737916Illumina Small RNA Adapter 2 (100% over 21bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA665380.13314928536666581RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC661560.13238486462949206RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCCGACTTAGAACTGGTGCTGGAATTCTCGGGTGCCAAGGAACTCC645770.1292251255090802RNA PCR Primer, Index 1 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC645300.12913107374298816No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA632290.12652764081350376No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC623660.12480069029994108No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC621830.12443448874260392RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG621540.12437645680182373No Hit
GACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAAC610730.12221326618170641RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTGGAATTCTCGGGTGCCAAGGAACT605210.1211086582054763RNA PCR Primer, Index 1 (100% over 25bp)
CAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAAG605160.12109865269844522No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG602380.12054234650751773RNA PCR Primer, Index 5 (100% over 45bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT588190.1177027836121001No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG574660.11499529340949259No Hit
GAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG571400.11434293435106684RNA PCR Primer, Index 1 (100% over 29bp)
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG568260.11371458850951564Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGA562720.1126059783304731RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG557100.11148135934018083Illumina Small RNA Adapter 2 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA554470.11095506967034655RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA545960.10925213237365845RNA PCR Primer, Index 1 (100% over 23bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC536150.10728905189416255No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG532060.10647060141902104No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGTGGAATTCTCGGGTGCCA525650.10518789541763787No Hit
CGACTCTTAGCGGTGGATCACTCGTGGAATTCTCGGGTGCCAAGGAACTC524580.10497377756717298RNA PCR Primer, Index 1 (100% over 26bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT523730.10480368394764478RNA PCR Primer, Index 5 (100% over 46bp)
CAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAAC521150.10428739978484158RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC511080.10227229066878409RNA PCR Primer, Index 1 (100% over 24bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG508830.10182204285238594No Hit
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG508580.1017720153172306RNA PCR Primer, Index 5 (100% over 45bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA504270.10090954061115237RNA PCR Primer, Index 1 (100% over 28bp)
AGTGGACGGGAGCGGCGGGGGCGGTGGAATTCTCGGGTGCCAAGGAACTC501100.10027519146538254RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)