FastQCFastQC Report
Fri 7 Jul 2023
SRR8302268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8302268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58472592
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC17665883.021224029199869No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT17230852.94682506976944No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC15584562.6652760664346813No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG14351822.4544525065692318No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC12171012.0814897345409284RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA7396061.2648763714801627No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT7155681.2237665126936736No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6458221.104486696946836No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA6069851.0380675445343692No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5626740.9622867411111174No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG5369540.9183003209435285No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC4182530.715297519220629RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG4033360.6897864216452043No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA3763220.6435869988455446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC3758340.6427524198003741No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC3697900.632415953101583No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC3606970.6168650775734381No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC3521380.6022274504266888No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA3502530.5990037178444219RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA3451850.5903364092359716RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC3420090.5849048046305182No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG3340770.5713394747405759Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3224260.551413900037132No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG3093820.5291060126084371No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2736690.46802953424743Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2722980.46568484598733023No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2410770.4122905993289985No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAA2295360.39255314695131016RNA PCR Primer, Index 1 (100% over 23bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG2153990.36837600768578893No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC2133940.3649470507481522No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGA2121820.3628742847589175RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA2098750.3589288465269335RNA PCR Primer, Index 1 (100% over 22bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC2039010.34871209403544146No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1988920.34014568740171464Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGTG1975270.3378112603593834No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG1808090.3092200872504506No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1778540.30416643749946987No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1751500.2995420486918042No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT1688920.2888395985592703No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC1668190.2852943478202574RNA PCR Primer, Index 8 (100% over 50bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1518520.25969774009676194RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1472990.25191118601344026No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1469260.25127328030883256No Hit
GCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCAAGGA1451110.24816926193386465RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1449820.24794864575184214No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA1434340.24530125156757204RNA PCR Primer, Index 1 (100% over 22bp)
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGC1417020.2423391800384016No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA1406420.2405263648993019RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG1403480.24002356522864593Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1388470.2374565505835623Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1362570.23302712491349792No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC1357920.23223188053644006No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT1332820.22793927110328888RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1306360.22341407406738528RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1269000.21702475580353953No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1268660.21696660890285144No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC1265640.21645012760850416RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC1239430.21196768564663596No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1228500.21009843380980958RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG1210610.20703888071183846No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG1205820.20621969349332078No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1182780.20227938587022107No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC1168800.19988852213016312No Hit
AGCAGCCGACTTAGAACTGGTGCTGGAATTCTCGGGTGCCAAGGAACTCC1152870.19716416881262935RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA1137550.19454413787574185No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1123850.19220115981860356No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGTGGAATTCTCGGGTGCCA1118810.1913392175260505No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG1039750.177818352913105Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA1039300.17774139377984133No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT1032030.17649807622689276RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1027650.17574900732979307No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG1021920.1747690610329024No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC984700.16840368561051647No Hit
CGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAA979290.16747846580839104No Hit
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT937540.1603383684444842RNA PCR Primer, Index 1 (100% over 30bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA934550.15982701775902117No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGGTGC934170.15976203004648742No Hit
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGTGGAATTCTCGGGTGCC925770.15832545955889898No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA906910.15510001677367066No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA882330.15089633789451304RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA882240.1508809460678603No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA879670.1504414239067767RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA865600.14803516834006608RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG859920.14706377305798243RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA841960.14399224853928144RNA PCR Primer, Index 1 (100% over 22bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA826580.14136195638462548RNA PCR Primer, Index 1 (100% over 32bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA825000.14109174431672195No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC817650.13983474514008204RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG815970.13954743104256437No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC815970.13954743104256437No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC813030.1390446313719084RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGACGGGAGCGGCGGGGGCGGTGGAATTCTCGGGTGCCAAGGAACTC810940.1386871989529727RNA PCR Primer, Index 1 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGA793230.13565842950830706RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA786340.13448009966789226RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG777730.1330076149181141No Hit
AGCAGCCGACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG776660.13282462320124272RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC775510.13262794986068002No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA759500.12988991491945492RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC751990.12860555249543237No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG751730.1285610872184356No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT744120.12725962276479894RNA PCR Primer, Index 8 (100% over 43bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAA737870.12619074591391466RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA735290.12574951354986966No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA732050.12519540779037125RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG725270.12403589018253201No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTG715270.1223256872211172No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT712830.12190839769853198RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT703030.12023239879634547No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAG701650.11999639078767023No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG687950.11765341273053194No Hit
CGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG677790.11591584652173449RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC667250.11411329260040327RNA PCR Primer, Index 1 (100% over 31bp)
GCCGCCGGTGAAATACCACTACTCTGATGGAATTCTCGGGTGCCAAGGAA666060.11390977844799492RNA PCR Primer, Index 1 (100% over 23bp)
ACGAGAACTTTGAAGGCCGAAGTGGAGATGGAATTCTCGGGTGCCAAGGA663460.11346512567802707RNA PCR Primer, Index 1 (100% over 22bp)
TGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA657000.1123603345649531RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA650220.11120081695711387RNA PCR Primer, Index 1 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC643880.11011654827957687No Hit
ACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTTGGAATTCTCGGGTGC642970.10996091981008813No Hit
GGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACT642820.1099352667656669RNA PCR Primer, Index 1 (100% over 25bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC637980.10910752853234212RNA PCR Primer, Index 8 (100% over 44bp)
CGAGAACTTTGAAGGCCGAAGTGGAGATGGAATTCTCGGGTGCCAAGGAA636720.10889204295920385RNA PCR Primer, Index 1 (100% over 23bp)
GCCGCCGGTGAAATACCACTACTCTGATCGTTGGAATTCTCGGGTGCCAA628020.10740416638277298No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG620470.10611296314690478No Hit
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTGGAATTCTCGGGTGCC618870.10583933067307841No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCC618370.10575382052500769No Hit
CAGTTGAACATGGGTCAGTCGGTCCTTGGAATTCTCGGGTGCCAAGGAAC588580.10065912590295296RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA585410.10011699156418445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)