FastQCFastQC Report
Tue 11 Jul 2023
SRR8310196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78058509
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC71131079.112532497898467No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC42677375.467356544050822No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG21283142.726562455862435No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT20233702.5921197136881005No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA13206981.6919334188153656No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG9167071.1743844607639122No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG8784391.1253596965322512No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC7294810.9345310451676704No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC7042950.9022655044564072No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC6906580.8847952758103539No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA6158320.7889364117882396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC6145890.7873440165248352No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC6092410.7804927455122157No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACTAGATCTCGTA5420630.694431660230661TruSeq Adapter, Index 22 (97% over 38bp)
CCGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT5209000.6673199458626605Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG3922960.5025666068000351No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG3895280.4990205488039747No Hit
ACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT3819140.4892663271341757Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT3769490.4829057137127741No Hit
TCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3394850.43491094609557557Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG2973620.3809475786938231No Hit
TCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA2573170.32964631696974894Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT2404680.3080612262271113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG2365860.30308803361847453No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACGT2318770.2970553793180959No Hit
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC2288400.29316470802689815No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1828140.2342012451198626No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1806500.2314289656749657No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT1683390.21565746278858594No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC1597110.2046042155378602No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACA1577240.20205868907898303No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT1488870.19073769395210968No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1460180.18706224583408324No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1357110.17385804794196108No Hit
TTCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1322140.16937807510517527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAG1249040.16001330489159102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACAC1186320.15197830642652937No Hit
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT1174170.15042178169198697Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACTCCA1157440.1482785175924895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCCCCCGCGGGGGCGCGCCGGCGTCTCCTCGAGATCGGAAGAGCACA1139510.14598152265501255No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1107480.1418781903712765No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1105050.14156688542436802Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1080650.13844102505211828No Hit
TCGGAGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1073540.13753016983708977Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1071660.13728932485758855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACA1057400.13546249006626557No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1050410.13456700793503498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGA1038750.1330732566260009Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC978160.12531113039835284No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC954010.12221729728401551No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA947010.12132053406246844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACAC914680.11717876906923753No Hit
CCGGGGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA890310.11405675196793727TruSeq Adapter, Index 22 (100% over 37bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA860730.11026728681174272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACTAGATCTCGTA847040.10851347416845998TruSeq Adapter, Index 22 (97% over 38bp)
TCGCGTGTGGTGTGCGTCGGAGGGCGAGATCGGAAGAGCACACGTCTGAA846850.10848913345244655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA828320.10611527309597985TruSeq Adapter, Index 22 (100% over 37bp)
TCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAAC820580.1051237091910121Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTC813930.10427178413054239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)