FastQCFastQC Report
Tue 11 Jul 2023
SRR8310197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85502113
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1015296211.874515896466793No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG35108424.106146476169542No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC24905002.912793511898355No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG21293062.4903548290087287No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA15224331.7805793875526794No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC15172801.7745526359097115No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC14758971.7261526624494063No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG12010671.4047220096186395No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT11002141.2867681995180633No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC7565520.8848342730430534No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG6370630.7450845103675976No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT6348780.7425290179670765No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG5850730.6842789955378061No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA5601490.6551288387457747Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG5081060.5942613371437967No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC4277090.5002320819837517No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG3570950.4176446493199531No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC3561400.41652771785885573No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC3488040.40794781293884513No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACA3216330.37616965091845156No Hit
TCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3009040.35192580562307274Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC2954850.34558795055743247No Hit
TCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA2738160.3202447172270468Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC2660020.31110576179561783No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACGT2557760.299145823448831No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC2556670.2990183412192398No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTA2454190.28703267251418685TruSeq Adapter, Index 13 (97% over 38bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACAC2330640.27258273722428356No Hit
TTCTCCCCCGCGGGGGCGCGCCGGCGTCTCCTCGAGATCGGAAGAGCACA2200110.2573164478403007No Hit
GCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC1908530.22321436664378105No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1906050.2229243153324176No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1839940.2151923426734495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1806260.21125325873525488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1792950.20969657206015482No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC1608680.18814505788880329No Hit
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT1580420.18483987641334665No Hit
TCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACAC1576440.18437439084107782No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT1565900.18314167276778295No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1537890.17986573033581052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT1526480.17853126039119055Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCCCGCGGGGGCGCGCCGGCGTCTCCTCGAGATCGGAAGAGCACAC1330660.1556289024108679No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA1329760.15552364185432468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACA1298400.1518558962396637No Hit
TCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACTCCA1263070.14772383461447322Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTC1224360.14319646112137605No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1166970.13648434629913767No Hit
TCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAAC1066590.12474428555935219Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC1030340.12050462425413977No Hit
TCGCGTGTGGTGTGCGTCGGAGGGCGAGATCGGAAGAGCACACGTCTGAA1000780.11704739975256517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGAGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG996660.11656554031594518Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTGGGAGGCCCCCGGCGCCCCCCCGGTGAGATCGGAAGAGCACACGTC995880.1164743145002744No Hit
TTCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC988570.11561936486879569Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGAGATCGGAAGAGCACAC974100.11392700903192884No Hit
TCGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC949090.1110019351217671No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG942030.11017622453377263Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG905900.10595059796943264No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG862920.10092382161362491No Hit
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA855960.10010980664302413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)