FastQCFastQC Report
Tue 11 Jul 2023
SRR8310198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71389844
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC67427079.444910679451828No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC42606275.968113615712622No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG28506543.9930805844035744No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT18870412.643290549843476No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG12741051.784714643724393No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC12026731.6846555933082021No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA10517361.4732291612795791No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG10002541.4011152622773626No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT6193570.8675701826719219No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC6078770.8514894639635296No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC5863940.8213969482830079No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG5536820.7755753045209064No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC3712110.5199773233851023No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA3583120.501908926989671Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC3144500.44046881514407005No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTA3143090.44027130805888864TruSeq Adapter, Index 6 (97% over 36bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACA3045710.42663071234614264No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC3033750.4249554040207736No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT3030950.4245631913693494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG2741810.3840616320719233No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC2361370.3307711388191295No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG2321970.3252521465098033No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACAC2097110.2937546690815013No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1802230.25244907384865556No Hit
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1770300.24797644886295028No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG1732400.24266757047402987No Hit
TCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA1729910.24231878136615626Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1612250.2258374454495236No Hit
GCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC1499420.21003267635659773No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC1496380.2096068454779086No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT1389970.19470136396431964No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1343720.18822285141847347No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA1260150.17651670453293047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACGT1203110.16852677251963177No Hit
TCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1120260.1569214803158836Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1098700.15390144289991725No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT1070100.14989527081751292No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC1044830.14635555163840952No Hit
TTCTCCCCCGCGGGGGCGCGCCGGCGTCTCCTCGAGATCGGAAGAGCACA1007690.14115313096916138No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT950500.1331421875638221No Hit
TCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAAC918840.1287073830837899Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT913550.12796638132449203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACAC882760.1236534429182952No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT874760.12253283534279749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC816990.11444064788823463No Hit
TCGCGTGTGGTGTGCGTCGGAGGGCGAGATCGGAAGAGCACACGTCTGAA794870.11134216794198346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGAGATCGGAAGAGCACAC785620.11004646543281422No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTC772710.1082380849578548No Hit
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT752400.10539314247555998No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA741120.10381308579410818Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGAGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG739660.10360857491157986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACA734050.1028227488492621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)