FastQCFastQC Report
Tue 11 Jul 2023
SRR8310199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65123339
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC704489510.817773026042168No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC50099897.693077592351338No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT23674763.6353725658937726No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG20948923.216806804086013No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG16729292.568862447301727No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC15891342.4401912193107913No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA13403862.058226774889414No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG11522381.769316527213078No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC8345601.2815067728637193No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG7520271.154773406197738No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC4935800.7579156836537512No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT4410170.6772026845859362No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC4268280.6554147968365075No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC3254850.4997977760323376No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTCGTA3235880.49688484185370163TruSeq Adapter, Index 4 (97% over 37bp)
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC3144870.4829098213161337No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACA3100710.4761288422265941No Hit
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG3050850.4684726008904427No Hit
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC2808230.43121714014080265No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC2501890.38417716880272373No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG2252910.3459450996516011No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC2238930.34379840382570065No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA2165890.33258276268666137Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC2102680.32287656503607715No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACAC1947370.29902797213760796No Hit
GCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC1881160.28886111014670174No Hit
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1677950.25765724328109163No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1639590.25176688191617447No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1542760.2368981725583819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT1514250.2325203257775219No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG1426890.21910578018734575No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG1383930.21250906683393492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA1303580.20017094025231108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1162450.17849975413576383No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1127970.17320518531766313No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT1074570.16500536005993183No Hit
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGAGATCGGAAGAGCACAC952590.14627474798243992No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACGT893640.1372226936951129No Hit
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT857930.1317392524974802No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA839010.12883399605784956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC819620.12585656887156846No Hit
TCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA812830.12481393191463969Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT808490.12414750416897388No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGT768670.11803295282510008No Hit
TTCTCCCCCGCGGGGGCGCGCCGGCGTCTCCTCGAGATCGGAAGAGCACA755370.1159906742496726No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC715700.1098991561228149No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT707910.10870296438577881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT703040.10795515260665611Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACAC677860.10408864324355359No Hit
TCGCGTGTGGTGTGCGTCGGAGGGCGAGATCGGAAGAGCACACGTCTGAA661600.10159184251900844Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC658060.1010482585974285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)