FastQCFastQC Report
Thu 13 Jul 2023
SRR8310200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56103407
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC759178613.531773569473241No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC578180510.305621902783908No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT29894755.32850883726188No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG24369854.343737983684306No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC20167793.5947531671294044No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG11912882.123379066800702No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG11127251.9833465728739077No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC10392081.852308185133926No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA9636181.7175748346263533No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC9157151.6321914282318006No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT9052911.6136114514400168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT6724151.1985279254074535No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG6420571.144417129604981No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC5611561.000217330829837No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT3761270.6704173955068361No Hit
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG2963280.5281818268184676No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT2220070.39571037102969525No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT2132470.3800963460204832No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC2078360.3704516554582862No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1884440.3358869096844689No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA1865420.33249674124068795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC1733610.3090026243860734No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC1713440.30540747730347284No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1649200.2939571922967174No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC1646850.29353832290434695No Hit
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1639750.29227280261250443No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCAGATCGGAAGAGCACACGTCTGA1623310.2893424992888578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC1525530.2719139677203561No Hit
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC1471190.2622282814303951No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1418540.2528438246183516No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTAGATCGGAAGAGCACACGTCT1363950.24311357775473422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG1320310.23533508401726833No Hit
CAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1137220.20270070229424747No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTCGTA1122400.2000591514878945TruSeq Adapter, Index 7 (97% over 36bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACA989240.17632440753553524No Hit
TCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA961090.17130688694182156Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGAGATCGGAAGAGCACAC824860.14702493914496137No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA808400.14409107097542223Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG784390.13981147348145898No Hit
GTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC752640.13415228062709275No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACAC715030.12744858792621988No Hit
CCGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT698060.12442381618642162Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCGTGTGGTGTGCGTCGGAGGGCGAGATCGGAAGAGCACACGTCTGAA669590.11934925805842772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA611920.10907002492736315No Hit
GTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG609440.1086279840366914No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCAGATCGGAAGAGCACACGTCTG587600.10473517232206593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAAC583270.10396338318633663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGGGCCCGGGGCGGGGTCCGCCGGCCAGATCGGAAGAGCACACGTCTG576310.10272281681574169Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGAGGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA572770.10209183909276667Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTAGATCGGAAGAGCACACGTC570590.10170327089048263No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACGT566200.10092078721707577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)