FastQCFastQC Report
Tue 11 Jul 2023
SRR8310201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83839256
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC46399925.534390715490128No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC45692095.449963678112792No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG26288933.135634934546652No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT20734262.47309685095488No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA14089361.6805206382079534No Hit
TCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGATCGGAAGAGCACACG13998571.6696915821867504No Hit
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA5687970.678437556745494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGCGGAGATCGGAAGAGCACACGT5474520.6529781228020439No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC4695370.5600443305460631No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACACGTCTG4463090.5323389320153318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG4184880.49915519288482235No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGATCGGAAGAGCACACGTCTGAA4148730.49484337027036596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACA4139700.49376630918575903No Hit
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG3448590.4113335643150268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGT3191630.38068443737143853No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC3096800.36937350684505116No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG2785400.33223100166824No Hit
AAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGAGATCGGAAGAGCA2719260.32434209578386525No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT2713320.3236335971302035No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGCGAGATCGGAAGAGCACACGTC2669750.31843674757800805No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC2483520.29622400275116945No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA2372590.2829927307561031Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT2133220.2544416663239473No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGATCGGAAGAGCACACGTC2132220.25432239045632754No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG2083600.248523197772652No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC2033630.2425629826676897No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG2025200.24155748710365466No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA2002350.23883203352854182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG1893200.22581307257783872No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT1882960.22459168769341178No Hit
TAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGAGATCGGAAGAGCACACG1795170.214120459275068No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT1783790.21276309990155448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGT1719390.20508173402683819No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCTAGATCGGAAGAGCACACGT1610020.19203653238525875No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1561280.18622302659746887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCGAGATCGGAAGAGCACA1549940.18487043825866012No Hit
TTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT1534710.18305386679481028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGATCGGAAGAGCA1432860.17090561967773185No Hit
TAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCAGATCGGAAGAGCAC1406910.16781041091299762No Hit
AAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAACTCCA1391010.16591392461784252Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC1375230.1640317514268018No Hit
TAAGCCATGCATGTCTAAGTACGCACGGCCGAGATCGGAAGAGCACACGT1360970.16233087755454317No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCAGATCGGAAGAGCACAC1301950.15529121584762154No Hit
GCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGATCGGAAGAGCACACG1297010.15470199306157964No Hit
TCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTCTGAACT1292770.15419626338287162Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGT1237960.1476587530786294No Hit
TCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGT1176420.14031851618530586No Hit
AAAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCT1121230.13373568105136813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCGGCGGCGACGTCGGCTAGATCGGAAGAGCACACGTCTGAACT1060770.12652426209507395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGGTCGGCGGCGACGTCGGCTACCCAGATCGGAAGAGCACACGTCTG1060050.1264383834703877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG1024860.12224106568884628No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1020950.1217746970464528No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC1006560.12005831731140362No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC997480.11897529243341569No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT985210.11751177753772052No Hit
CCCCCCGGGGCCGCGGTTCCGGGGTAGATCGGAAGAGCACACGTCTGAAC969600.11564988124417516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAATCCCGCCCAGGCGGAACGATACGGAGATCGGAAGAGCACACGTCTG946840.1129351624971481Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGACCGTCGTGAGACAGGTTAGTTTTACCCTAGATCGGAAGAGCACACG931630.11112097655065069No Hit
CCGGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTG923610.11016438409233975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCCCGGGGCCGCGGTTCCGGGGCAGATCGGAAGAGCACACGTCTGAAC902300.10762261535336144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTG900900.10745562913869369Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCCGGCGGCGGGTCCGGCCGTGTCGGCGAGATCGGAAGAGCACACGT896950.10698448946159542No Hit
TAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA885160.10557822698235776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTGAA883100.10533251869506094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG854850.1019629754348011No Hit
AAATGTGAATTGCAGGACACATTGATCAGATCGGAAGAGCACACGTCTGA853720.10182819370439068Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC839610.10014521121227507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)