FastQCFastQC Report
Mon 17 Jul 2023
SRR8310202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59399409
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC52254418.797126247501891No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC47375127.9756887816846795No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG26002734.377607528047965No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT24329544.095922907246434No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA15018142.528331552928414No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG6780641.1415332432011234No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC4325520.7282092655164296No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC3440730.5792532380246409No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG3311690.5575291161566944No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT3193470.5376265612339678No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT3024240.5091363787811425No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC2814220.47377912463741856No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG2482420.4179199830085851No Hit
CCCCCCGGGGCCGCGGTTCCGGGGTAGATCGGAAGAGCACACGTCTGAAC2245720.3780711016838568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC2218280.3734515271018942No Hit
ACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT2060490.3468872897371757Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1465190.2466674373814056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCGGGGCCGCGGTTCCGGGGCAGATCGGAAGAGCACACGTCTGAAC1314620.22131870032578946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG1282760.2159550105961492No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1275600.21474961139764875No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1265180.2129953851897752No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1198380.2017494820529275No Hit
CCCCCCGGGGCCGCGGTTCCGGGGAAGATCGGAAGAGCACACGTCTGAAC1163380.1958571675351181Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1110760.1869984935372Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA1053910.17742769124184382Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACAC1020960.17188049800293467No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT994560.1674360093380727No Hit
CAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT994310.16739392137723122No Hit
AAGTGGGAGGCCCCCGGCGCCCCCCCGGTGAGATCGGAAGAGCACACGTC924650.15566653196835678No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT890760.14996108799668362Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT882970.14864962713686258No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA808830.13616802146970858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC797000.13417641916268896No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGCGGAGATCGGAAGAGCACACGT770230.1296696403157816No Hit
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT764660.12873192054823307Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTC761410.12818477705729361No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT756600.12737500469070323No Hit
TTTCGCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC752440.12667466102230074No Hit
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT748460.12600462068570414No Hit
TTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAACT688600.11592707934181634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACAC673720.1134220039125305No Hit
TTGGGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC673080.11331425873277627No Hit
TCCCCCGGGGCCGCGGTTCCGGGGTAGATCGGAAGAGCACACGTCTGAAC672540.11322334873735865Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGAGATCGGAAGAGCACACG672290.11318126077651716No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG671520.11305162985712534No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC664790.11191862195127228No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC655710.11038998721350914No Hit
AGGGTCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC650950.10958863243908706No Hit
TCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGATCGGAAGAGCACACG648220.10912903190669791No Hit
TCGTGGCGCAATGAAGGTGAAGGCCGGCGCGAGATCGGAAGAGCACACGT646160.108782227109364No Hit
TTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGAGATCGGAAGAGCACA642730.10820478028661867No Hit
TCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGA638780.10753979050532303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTCACGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC636500.1071559483024486No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTA614140.1033916010847852TruSeq Adapter, Index 13 (97% over 38bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCCAGATCGGAAGAGCACACGTCTGA595690.1002855095746828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)