FastQCFastQC Report
Mon 17 Jul 2023
SRR8310203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85028216
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC64254377.556829135401359No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC47607625.599037853505006No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG41435654.873164691588966No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT19753922.323219388726208No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA17362142.041926882248123No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG11750151.3819118585294086No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC4775670.5616570856902372No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT4426660.5206107111549888No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG4379740.515092542927162No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC3736110.4393964939826563No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG3535190.41576669090646334No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC3277670.38548027398340334No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT2700990.3176580818771971No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC2161760.2542403100636617No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC2021830.23778342003553266No Hit
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG1928210.22677295734394803No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1879700.2210677923667127No Hit
CCCCCCGGGGCCGCGGTTCCGGGGTAGATCGGAAGAGCACACGTCTGAAC1689110.19865288012158225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC1632990.19205271812359323No Hit
CCCCCCGGGGCCGCGGTTCCGGGGCAGATCGGAAGAGCACACGTCTGAAC1506230.177144725699055Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1361920.16017271254991403No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1263470.14859420312899427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA1262080.148430727983285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA1173550.1380188901058444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT1107750.13028028248881524No Hit
TTTCGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1105370.13000037540479506No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1097100.1290277570918341Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC1087580.12790812875575328No Hit
TTTCGCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1036540.12190541549172336No Hit
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT1012070.11902754727912909Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC942690.11086790295588467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)