FastQCFastQC Report
Tue 11 Jul 2023
SRR8310204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60811836
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC843453013.869882172279752No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC649131910.674433509950267No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG57514299.45774602167907No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT31748575.220787940031937No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA20774133.416132675224606No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC10598851.7428926171543315No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT7269241.1953659810567139No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG7063051.1614597526705164No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG6361831.0461499633064852No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC4462560.7338308285906711No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT3822940.6286506462327498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT3681020.6053130841173748No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC3232600.5315741494797164No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC2622140.43118908628247965No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT2551390.41955483797594934No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1962080.3226477161452583No Hit
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC1742700.2865725021030446No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG1650660.27143729059586363No Hit
TTTCGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1480850.24351345024346904No Hit
TTTCGCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1369340.22517655937899983No Hit
TTGGGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1275980.20982428486454513No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC1272790.20929971593030014No Hit
CAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1169470.19230960236096145No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT1158100.19043990054830773No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1134160.18650316691638777No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA1131190.1860147751500218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTC1032600.16980247069008078No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC1021820.16802978946401156No Hit
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG933800.1535556334789826No Hit
AGGGTCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC905520.14890522298981404No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC850740.1398971081879521No Hit
TTCTATCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG791190.13010460660980536No Hit
AGGGTCACGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC790740.13003060785732568No Hit
AGGGGCACGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC725950.11937643191697091No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTAGATCGGAAGAGCACACGTCT705750.11605471013899334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTC696150.1144760700860931No Hit
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT695680.11439878250016987No Hit
TTTGGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC695510.11437082741589975No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCAGATCGGAAGAGCACACGTCTGA658780.10833088479683461Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT646630.10633291847988277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT619500.10187161591371785Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)