FastQCFastQC Report
Tue 11 Jul 2023
SRR8310205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8310205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55472833
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC615287011.09168158042334No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC614433211.07629026265884No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACACG33888056.108945256140064No Hit
TAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT29472555.312970044273744No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACA18936593.4136691738819254No Hit
CGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC5686901.0251684820207396No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACG4679420.8435516534733317No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG4661020.8402347145313455No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACACGT4118050.7423543701112219No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTC3871670.6979398366043429No Hit
TCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACAC3570640.6436736339029233No Hit
AAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT2795830.5039998588137728No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTC2330320.4200831062657283No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT2096100.37786063675529247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACG1705130.3073810922907074No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAAGATCGGAAGAGCACAC1696990.30591370734572004No Hit
TTTTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGT1566700.2824265348048837No Hit
TCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGAGATCGGAAGAGCAC1463640.26384807136134547No Hit
TTTCGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1071820.19321529873911433No Hit
GAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1067090.19236262910891896No Hit
CAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGT1021930.18422170722739184No Hit
TTTCGCTCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC1011610.18236133712514738No Hit
TCGGCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGA1006240.1813932957056655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCCCGGGGCCGCGGTTCCGGGGTAGATCGGAAGAGCACACGTCTGAAC952550.17171468419505453Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG928880.16744773067566243No Hit
CCCCCCGGGGCCGCGGTTCCGGGGCAGATCGGAAGAGCACACGTCTGAAC923740.16652115099295542Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGCGCGGGGCCACGCGCGCGTCCCCCGAAGAGATCGGAAGAGCACAC921170.1660578611516019No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC883810.15932303295200373No Hit
AGGGTCCCGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC870470.1569182522190637No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCCTAGATCGGAAGAGCACACGTCT857100.15450806343350088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACAC817900.14744154133970408No Hit
CCCCCCGGGGCCGCGGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCA810840.14616884628913762Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGTCACGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC765190.13793959288143803No Hit
TTTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGT758450.1367245837255148No Hit
TCGGCGGCGGCGGCGGCGGGACCGAGATCGGAAGAGCACACGTCTGAACT747120.13468214251830263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAAGATCGGAAGAGCACACGTC686300.12371821716767195No Hit
AGGGGCACGGGGCGGGGTCCGCCGGCCCTGAGATCGGAAGAGCACACGTC670610.12088980564594563No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTCGTA560360.10101521225714216TruSeq Adapter, Index 7 (97% over 36bp)
TGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGT556160.10025808488994964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)