FastQCFastQC Report
Mon 17 Jul 2023
SRR8353546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110764990
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA31062712.8043797954570304No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA23981022.1650360822494545No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA17883161.614513755655104No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA14894891.3447290520226651No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT6458440.5830759340112792No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT5537690.49994948764948205No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT5368040.484633276272584No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA5036170.4546716430886691No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT4901770.44253784521625467No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC4466920.403279050537539No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG3845130.3471430819431302No Hit
TACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAG2334310.21074438773478876No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2119450.1913465617610763No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATC2071330.18700222877282793No Hit
ACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATCGGAAGAG2067200.1866293672757069No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT1922650.17357921487646955No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC1652560.1491951563395618No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACCA1485340.13409832836169625No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAA1481740.13377331591868513No Hit
ACGATGGATACTAGGCGCTGTGCGTCTGTAGGCACCATCAATAGATCGGA1466670.132412777719747No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGTAGGCAC1438620.12988038910128552No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC1365450.12327451119708492No Hit
GAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATCG1349420.12182730301334385No Hit
TCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTGTAGGCACCATCAATAG1335930.12060940916439392No Hit
ACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGAAGAGC1335400.12056156011028396No Hit
ATGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA1325130.1196343718353606No Hit
CGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA1308330.11811764710130883No Hit
GGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA1296110.11701441041975448No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA1236520.11163455167557909No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGACTGTAGGCACCATCAATA1217180.10988851260673611No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCCTGTAGGCACCATCAATAGAT1174140.10600280828807007No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCAATCTGTAGGCACCAT1154990.10427392265371938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)