FastQCFastQC Report
Thu 13 Jul 2023
SRR8353548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138823783
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA46451383.3460678708056815No Hit
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA34126432.4582552976531407No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA14419641.0387009839661263No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA11766290.8475701890359809No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT7211390.5194635849968157No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT7101890.5115758875408258No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT6066210.43697195602283795No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG3875070.27913588840897674No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT3727050.26847345025887964No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA3563470.256690166698598No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC3448150.24838323272028973No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC2998980.21602782572205226No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACCA2773910.19981518584607366No Hit
TCGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2267280.1633207186120263No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2224250.16022110563000577No Hit
TACCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA2118390.15259561108488162No Hit
CGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA2013300.1450255825401329No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT1779740.12820137598468986No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGCTGTAGGCACC1635000.11777520858943888No Hit
TACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAG1612190.11613211837052445No Hit
TCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTGTAGGCACCATCAATAG1595360.1149197900766038No Hit
ATGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA1448650.1043517161609117No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATC1422490.10246731282348069No Hit
GGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA1402550.10103095951505658No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGCTGTAGGCACCAT1389000.10005490197598202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)