FastQCFastQC Report
Fri 14 Jul 2023
SRR8353549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134926682
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA25924741.9213946134093773No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA12588150.9329622438947992No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA9256980.6860748269197045No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT6239710.4624518966530282No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT5854380.43389342368917067No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC3288440.2437205118554683No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA3078020.22812537552802195No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC3048410.22593085035619567No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA2790280.20679971956918056No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACCA2769520.20526110617616758No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGCAC2451490.18169052730430293No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAA2176940.1613424392960319No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG2148370.15922499302250684No Hit
TCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTGTAGGCACCATCAATAG2099570.15560821394837235No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG1998300.14810265622629037No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT1827530.1354461528965783No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT1783670.13219549858937463No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGTAGGCAC1745090.12933616791970026No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA1690330.12527766746683952No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCCTGTAGGCA1668360.1236493757402261No Hit
TTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCA1638010.12140000596768546No Hit
TCGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG1637120.12133404421817769No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCAATCTGTAGGCACCAT1591060.1179203383953368No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1561590.11573618922905107No Hit
CCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC1551920.11501950370350024No Hit
TAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACC1415110.10487992286062442No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC1392760.10322346769040093No Hit
TCGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA1377380.10208358936744624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)