FastQCFastQC Report
Mon 17 Jul 2023
SRR8353550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155654722
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA32101102.062327412078125No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA17156591.1022209785579136No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA15774951.0134578506394427No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGCAC15053500.9671084697321293No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT8316630.5342998845868615No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT7573070.48653005207256095No Hit
TCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGC6401430.4112583234063404No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC5103520.3278744091040168No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC4888400.3140540766890451No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT4049980.2601899863982282No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA3809210.24472177593173175No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA3685590.23677983890524051No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAA3529920.22677885737382256No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCAATCTGTAGGCACCAT3354030.21547884682868793No Hit
CCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGC3222680.2070402978201972No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT2982340.19159971259978867No Hit
TCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCTGTAGGCA2902520.18647169598876673No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG2797970.1797549065038965No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2780560.17863640526112662No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGTAGGCAC2543190.16338662697299988No Hit
TCGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA2532790.16271848148622178No Hit
TCGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2468900.15861388387562056No Hit
TTACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATA2262050.1453248556121542No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA2203150.14154083934568976No Hit
TTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCA2199960.14133589856657225No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCCTGTAGGCA2157910.138634406478205No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT2007800.12899062580317994No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGCACC1876910.12058162938352747No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCCTGTAGGCACCATCAATAGAT1754810.11273734438971919No Hit
TTTTCGATCCTATTGTGTTGGCCTTCGGGATCGGCTGTAGGCACCATCAA1698580.10912486162803336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)