FastQCFastQC Report
Fri 14 Jul 2023
SRR8353551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100858438
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA23702362.3500621732809304No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA11983711.1881712861743903No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA11154261.1059322572495125No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGCAC7894810.7827614780232864No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT5712690.5664067492300446No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT4888830.4847219624797282No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC3469150.34396229693741637No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC3412810.3383762496896888No Hit
TCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGC3186670.31595472458139795No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA3059090.30330531194623495No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA2911250.2886471432365431No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2902310.28776075235271836No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA2698940.2675968469787327No Hit
TCGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG2625430.26030841365994584No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT2585220.25632163766010335No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCAATCTGTAGGCACCAT2196700.21780031929504998No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG2186830.21682171996357907No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGTAGGCAC2121020.21029673293175533No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAA1978690.19618487448715No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT1923750.1907376356552339No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCCTGTAGGCA1837730.18220884999230305No Hit
TCGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAGA1782410.17672393459038102No Hit
TCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCTGTAGGCA1756810.17418572355839976No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1679690.16653936282455614Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT1650840.16367891797015535No Hit
CCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGC1634820.16209055309779832No Hit
TTACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATA1589510.15759811786892833No Hit
ACGATGGATACTAGGCGCTGTGCGTCTGTAGGCACCATCAATAGATCGGA1302560.12914735007099754No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCCTGTAGGCACCATCAATAGAT1246620.12360096237064469No Hit
TTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCA1133080.11234359984833395No Hit
ATGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA1109040.10996006105111404No Hit
TACCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA1092260.10829634303874507No Hit
TACGCTTGTGGAGACGTCGCTGCCGTGATCGCTGTAGGCACCATCAATAG1052850.104388886133652No Hit
TTTTCGATCCTATTGTGTTGGCCTTCGGGATCGGCTGTAGGCACCATCAA1028660.10199047500616656No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCACTGTAGGCACC1016730.10080762900571592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)