FastQCFastQC Report
Fri 14 Jul 2023
SRR8353556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149547292
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA40751532.724992840391921No Hit
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA30423812.034393909319334No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA19496051.303671215925461No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA9350690.6252664207386651No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT8732840.5839517308009831No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT7382250.49363983133843703No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT6729020.44995933460299636No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA6429310.4299181826709373No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG5333160.3566202990823799No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT4056380.27124396207722706No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC3759430.2513873671480457No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACCA3706190.24782728930992615No Hit
GGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA2994210.2002182694154034No Hit
TCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTGTAGGCACCATCAATAG2965570.19830315616815047No Hit
CGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA2597990.17372364054576125No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAA2590530.17322480169015697No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT2467480.1649966353118584No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC2333440.1560335843460141No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC2061140.1378252974316646No Hit
TTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACC2049290.13703290595191786No Hit
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGCTGTAGGCACC2023950.13533845868636657No Hit
CCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC1969180.13167607207491258No Hit
ACGGGCCAAGGGACAGTCTCAGGTAGACAGTTCTGTAGGCACCATCAATA1921290.12847374060106684No Hit
AGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGCTGTAGGCACCAT1909880.12771077125221367No Hit
TACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAG1854290.12399355248773078No Hit
GCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCAC1840210.12305204429913716No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACTGTAGGCACCATCAATAGA1637550.10950047828348507No Hit
TGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCTGTAGGCACCA1620590.10836638887449732No Hit
ATGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA1577680.1054970624275831No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATC1553080.10385209783671642No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)