FastQCFastQC Report
Fri 14 Jul 2023
SRR8353559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8353559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116121741
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA72312406.227292096834821No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA28327082.4394294949470314No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGA22104821.9035901296037234No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT14880311.2814404840864382No Hit
ACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAGA14423181.2420740402092316No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC11357370.9780571581337211No Hit
GTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA7847210.675774401281152No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGAT7303120.62891926499793No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAG7253430.6246401352180898No Hit
ACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGAAGAGC6237860.53718278302424No Hit
ACCTGCGGAAGGATCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCA4853980.4180078560826951No Hit
ACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATCGGAAGAG3993140.34387531272029415No Hit
ACGATGGATACTAGGCGCTGTGCGTCTGTAGGCACCATCAATAGATCGGA3429790.29536157230022925No Hit
ATGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATA3171270.2730987300646827No Hit
CCTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAAT3146050.27092687148050937No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGAT3010390.259244304647482No Hit
ATCTCTCAGGCGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2743790.23628564094642707No Hit
TTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2661520.22920083500987126No Hit
ACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATCGGAAGAGC2268520.19535704343254723No Hit
TACGGCGAGACGAGCCGTTTATCCTGTAGGCACCATCAATAGATCGGAAG2106250.18138291605531476No Hit
TATTCTGGTGTCCTAGGCGTAGAGGCTGTAGGCACCATCAATAGATCGGA2080830.17919383416753976No Hit
TCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGAAGAGCACA1998320.17208836026666188No Hit
GAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATCG1996780.17195574082892884No Hit
TCGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG1930800.1662737729707308No Hit
AAGATGAGTGCTCTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1829100.1575157230892706No Hit
AGGTCGACGCGGGCTCTGCCCGTTGCCTGTAGGCACCATCAATAGATCGG1772310.15262516603157028No Hit
TCGGCTGTCAGCGGACTGCTCGAGCTGCCTGTAGGCACCATCAATAGATC1760260.15158746198956832No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1686490.1452346464560844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGTAGGCACCATCAATAGATC1685130.1451175279915929No Hit
TAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAA1673220.14409188026211214No Hit
TGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCG1667030.1435588190156398No Hit
TCCTGCGGAAGGATCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCA1508300.12988954411215725No Hit
ACGGCGTGCCTCGGCATCAGCGTGCTCCTGTAGGCACCATCAATAGATCG1306870.11254309388971356No Hit
AGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGA1286470.11078631692234102No Hit
AAGTCTGGTGCCAGCAGCCGCGGTACTGTAGGCACCATCAATAGATCGGA1276390.10991826242081576No Hit
ACCCCACAGCCTGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1275220.10981750609474585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)