FastQCFastQC Report
Tue 27 Jun 2023
SRR836194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR836194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140401605
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length44
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCAC99402647.079879179443853No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCACC71610345.100393261173902No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCACCA40372492.875500604141954No Hit
GGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAACTGTAGGCAC19490471.388194244645565No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACC18175251.294518677332784No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCA14930451.0634102081667798No Hit
GGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGACTGTAGGCACC13024250.9276425294425943No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCCTGTAGGCA12763890.9090985818858696No Hit
GGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGCTGTAGGCA11948390.8510152002891991No Hit
TGGATAACTGTGGCAATTCCAGAGCTAATACATGCCTGTAGGCA10510120.7485754881505806No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGCTGTAGGCAC8747320.623021367882511No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCCTGTAGGCAC8557600.609508701841407No Hit
GCTTGCTCTCCGGGGCCGCGGGTGAGTGTCCTCCGCTGTAGGCA5248540.373823361919545No Hit
TGGATAACTGTGGCAATTCCAGAGCTAATACCTGTAGGCACCAT4811990.34273041251914466No Hit
GCTGCGATCCATTGAAAGTCAGCCCTCGATCCAACTGTAGGCAC4799770.34186005209840725No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTCGGCTGTAGGCACCATC4418660.31471577550698226No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCGCCTGTAGGCACC4025260.2866961527968288No Hit
TGCTGCGATCCATTGAAAGTCAGCCCTCGATCCAACTGTAGGCA3820710.27212723102417524No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGCTGTAGGCA3744290.26668427330300104No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTCGGCTGTAGGCACCAT3501240.24937321763522577No Hit
TTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCTGTAGGC3298690.2349467443766045No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTGCCTGTAGGCACCA3253060.2316967815289576No Hit
CTTGGATAACTGTGGCAATTCCAGAGCTAATACTGTAGGCACCA2841130.20235737333629483No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGTAGGCACCA2659870.18944726450954746No Hit
GCTGCGATCCATTGAAAGTCAGCCCTCGATCCCTGTAGGCACCA2418810.1722779451132343No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGTCCTCCGCTGTAGGC2328840.16586989870949123No Hit
GCTTGCTCTCCGGGGCCGCGGGTGAGTGTCCTCCGCCTGTAGGC2078150.14801468971811255No Hit
CCATCTAAGGCTAAATACCGGCACGAGACCGACTGTAGGCACCA1799420.128162352560001No Hit
GCTGCGATCCATTGAAAGTCAGCCCTCGATCCACTGTAGGCACC1771870.12620012427920607No Hit
TGGATAACTGTGGCAATTCCAGAGCTAATACACTGTAGGCACCA1686300.12010546460633409No Hit
CTGGGTCGGGGTGTCGTGCGTGGAAGAGCAGCTCTGTAGGCACC1644770.11714752121245338No Hit
TGCTGCGATCCATTGAAAGTCAGCCCTCGATCCCTGTAGGCACC1523590.1085165657472363No Hit
TGGATAACTGTGGCAATTCCAGAGCTAATACATGCCCTGTAGGC1514490.10786842500838933No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTCGGGCTGTAGGCACCAT1477240.10521532143453773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)