FastQCFastQC Report
Tue 27 Jun 2023
SRR836199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR836199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121349883
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length44
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGCTGTAGGCACC87523257.212470901187437No Hit
CGTTGAACCCCGCTCGTGATTGGGACTGGGGCTCTGTAGGCACC29066522.3952655974130606No Hit
GTTGAACCCCGCTCGTGATTGGGACTGGGGCTCTGTAGGCACCA16801541.3845534568830198No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGACTGTAGGCAC16633551.3707100154352847No Hit
CTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGACTGTAGGCACC16160381.331717806435792No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCA13888161.1444724672705289No Hit
CGCCCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGCTGTAGGC13838641.1403917051984302No Hit
GTTGAACCCCGCTCGTGATTGGGACTGGGGCTTCTGTAGGCACC9147500.753812016448339No Hit
TGGATAACTGTGGCAATTCCAGAGCTAATACATGCCTGTAGGCA7818010.6442536083862561No Hit
CGCCCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGCTGTAGGCA7339500.6048213495187301No Hit
CCGTTGAACCCCGCTCGTGATTGGGACTGGGGCTCTGTAGGCAC7025210.5789218601883613No Hit
CGTTGAACCCCGCTCGTGATTGGGACTGGGGCTTCTGTAGGCAC6728130.554440584009463No Hit
TCGGTCGGTGCGGAGAGCCAGCCGTGACGGGACTGTAGGCACCA6191710.5102361738577037No Hit
CGCCGCGGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTAGG5658200.46627156616211984No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACC5537470.4563226484528214No Hit
GGTCGGCCCGTCCGGTGCGCTCGGATCCCCCCTGTAGGCACCAT4969110.4094861797270954No Hit
CTCCCGGGGCCACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT4851520.39979601793270786No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCAC4569060.3765195224786496No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCGCCTGTAGG4404420.36295214227771444No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCACC4164780.343204286402155No Hit
GGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTAGGCACCAT3920810.3230996110643139No Hit
GCGCCGCGGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTAG3863900.31840986612240907No Hit
CGGTCGGTGCGGAGAGCCAGCCGTGACGGGACTGTAGGCACCAT3531920.2910526085962522No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTCGGCTGTAGGCACCATC3481760.2869191064650635No Hit
CCCGTTGAACCCCGCTCGTGATTGGGACTGGGGCTCTGTAGGCA3336320.27493392803683214No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATC3072620.25320337556485323No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCGCTGTAGGCACCA2927680.24125940030778606No Hit
GCGGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTAGGCACC2756140.22712341634478542No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCGCTGTAGGC2708960.22323548511373514No Hit
TTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCTGTAGGC2571670.2119219183754796No Hit
GTGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGCTGTAGGCAC2477550.2041658334355378No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCGCCCCTGTA2428490.20012297828091025No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTGCCTGTAGGCACCA2351770.19380076369748128No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTCGGCTGTAGGCACCAT2178560.17952716114279238No Hit
CGACCTCAGATCAGACGAGACAACCCGCTGACTGTAGGCACCAT2001010.16489591506239853No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGTAGGCACCA1964380.16187737074291206No Hit
CCGGTACGCACCGCATGTTTGTGGGGAACCCGCTGTAGGCACCA1953750.16100139132396196No Hit
CGCCGCTGAATACCGCAGCTAGGAATAATGGAACTGTAGGCACC1887390.15553290644705442No Hit
AGCGCCGCGGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTA1781760.1468283245069136No Hit
CGGGGCTTCGATTGGCCTGGGATAGCCGGCCGCTGTAGGCACCA1648300.13583037405977555No Hit
CGTTGAACCCCGCTCGTGATTGGGACTGGGGCCTGTAGGCACCA1634920.13472777719942258No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATC1558970.12846901549958642No Hit
CGCCCTCGGTCGGTGCGGAGAGCCAGCCGTGACGCTGTAGGCAC1508160.1242819492458843No Hit
CTGGGTCAGGGTTTCGTGCGTGGCAGAGCAGCTCTGTAGGCACC1478970.12187650811332056No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCGCCCTGTAG1467970.12097003834770899No Hit
GAAGAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCCTGTAGGCA1442420.11886455630122034No Hit
CCCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGCTGTAGGCAC1439390.11861486508396551No Hit
GCCCTCGGTCGGTGCGGAGAGCCAGCCGTGACGGGCTGTAGGCA1421320.11712578247809272No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCCTGTAGGCA1309950.10794818813298732No Hit
CGCCGCTGAATACCGCAGCTAGGAATAATGGACTGTAGGCACCA1294740.10669478766617352No Hit
CGACCTCAGATCAGACGAGACAACCCGCTGAACTGTAGGCACCA1290150.10631654255488653No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCCCCCGCCCCCTGT1284020.10581139167641389No Hit
GGGCTGCACGCGCGCCACAATGGGCGGATCAACGCTGTAGGCAC1281150.1055748854739316No Hit
CGGCGCAAGACGGACGAAAGCGAAAGCATTTGCCTGTAGGCACC1251660.10314472243866935No Hit
CCCCGAGAGAGGGGCCCGCGCCCTGGAAAGCGCCGCCTGTAGGC1227590.10116120177882659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)