FastQCFastQC Report
Tue 11 Jul 2023
SRR8363858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8363858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23122309
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7940953.4343239682507485No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7934933.431720422039166No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6373652.7564937394444473No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3332191.4411147260422823No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2861401.2375061677447525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2256730.9759968176188633No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1867960.8078604952472525No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1644730.7113173688665781No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1623690.7022179316088198No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA1302320.5632309472207122No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1289440.5576605692796511No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1211670.5240263850811785No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1211460.5239355637017047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT1209730.5231873685279441No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1125440.48673339673818905No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1118800.48386171121577864No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1082190.46802851739417545No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1049970.45409392288633454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1011380.43740441320112106No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT979340.42354766558997203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG972730.4206889545503436No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC837910.362381628928149No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC828880.3584763096107746No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT776190.33568879301803295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT762430.3297378302487005No Hit
CGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACG753190.3257416895518523No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC749950.324340445411399No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA713120.308412105382728No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG703380.30419972330618017No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG688240.29765193432887693No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG670540.2899969894875118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG656460.2839076322351717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG635320.27476494670147344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA626460.2709331494531969No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGT617010.2668461873768749No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCAGATCGGAAGAGCA595360.2574829356358831No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC590120.2552167259766315No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC583210.2522282701091833No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC562770.2433883225070645No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT557840.24125618250322667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC553450.23935758318946432No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG545660.2359885424937449No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT516070.22319137764312377No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT491150.21241390727889678No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC485160.20982333555009577No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT438700.1897301865484109Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT395430.1710166575492093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA363190.15707341338618042No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCAC352850.15260154165399312No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA351080.1518360471698566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG347570.15031803268436555No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCGAGATCGGAAGAGCACACGT346470.14984230164902648No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC344670.14906383268210802No Hit
GGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGACAGATCGGAAGAGCACAC340480.14725172992022553No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCAGATCGGAAGAGCACACGTC339340.1467586995745105No Hit
GGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGT327950.14183272094495405No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG318600.13778900714457196No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC313150.13543197610584654No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT306510.1325602905834361No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG304520.1316996498922318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA304360.1316304526507279Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCCAGATCGGAAGAGCACACGT283400.12256561401372155No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA279870.12103894987304253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGCAGATCGGAAGAGCACACGTCT279660.12094812849356869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT277390.1199663926297326No Hit
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT269610.11660167676160715No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG263390.1139116339981444No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCAC257260.11126051468302754No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG255540.110516644336861No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG254080.10988521950813822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCACACGTCTGAA253780.10975547468031847Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC253360.10957383192137084No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT250570.10836720502264718TruSeq Adapter, Index 7 (100% over 48bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT243260.10520575605143932No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT238840.10329418225489505No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC234840.10156425121729841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)