FastQCFastQC Report
Tue 11 Jul 2023
SRR8363859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8363859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21891024
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT10495484.794421677122093No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC9690394.426649936521928No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6193302.829150431702053No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT5650662.5812680119486413No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3054071.395124321274327No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2630971.201848757737418No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2502751.1432768060553038Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2452711.120418122057698No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2440571.114872470104642No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT2368641.0820142538786672No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA1875430.8567118651005087No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTC1874770.8564103716664877No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1828060.8350728590859888No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1671150.7633950791886209No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1502690.6864411641958823No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1496670.6836911786310225No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1464180.66884947912898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1417250.6474114687371408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1401120.6400431519329566No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1386310.6332778219968148No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACA1335110.6098892404485053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1203430.5497367322789468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1172570.53563963019729No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1166550.5328896446324302No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC1161930.5307791905942819No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1152600.5265171697769825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1113460.50863769552306No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1091210.4984737123306795No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT997660.4557393020993445No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT993890.4540171350595568No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC920240.4203732086721937No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGT913000.4170659170626281No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT902090.4120821392366113No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT884090.40385959103603375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA788060.35999229638595254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT713340.32585958518888836No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC691680.3159651188541934No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC607100.2773282784761462No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG605340.27652429598542305No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC574850.26259621295011143No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG543130.2481062557877603No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC521360.23816154054739513No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG474890.21693366194290412No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCAGATCGGAAGAGCA461700.2109083613448142No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC453470.2071488295842168No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT447870.20459070347737043No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCAGATCGGAAGAGCACA427600.19533120058705342No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG404490.18477436231397854No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC393040.1795439080419445No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG375450.17150865121704678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC365380.16690859230705699No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAA365320.16688118381305508Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTC356110.16267397998375954No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC354730.16204358462171528No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCGAGATCGGAAGAGCACACGT351410.16052698128694207No Hit
GTCCCCCGCCTCCCCTCCGCGCGCCGGGAGATCGGAAGAGCACACGTCTG338440.1546021785001926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCAC335440.15323175380009632No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCCAGATCGGAAGAGCACACGT326650.14921640942881428No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG315600.1441686784501264No Hit
TGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGT313250.14309517910171765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG308540.14094361232256652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT298730.1364623235532518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC296650.13551216242785172No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG295900.13516955625282764No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT289100.13206326026594278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT287910.13151965846823793No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAGATCGGAAGAGCACACGT287490.13132779901022446No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACAGATCGGAAGAGC277850.12692416764058181No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT274350.12532533882380284No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT272850.12464012647375472No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG272550.12450308400374509No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG269150.12294993601030266No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTC266390.1216891452862141No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG264580.1208623223838227No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACAC263190.12022735893944478No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG260810.11914015534403508No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA248950.1137224096963212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT244980.11190888100986048No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC242210.11064352220343826No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT235160.10742302415821206No Hit
TCGTGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTC225400.10296457580056556No Hit
GGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACACGTCTG223440.10206923166316935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA222520.10164896808847317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG220960.1009363472444231No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC219480.10026027105904228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)