FastQCFastQC Report
Tue 11 Jul 2023
SRR8363860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8363860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492669
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9639124.103033163239137No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA4471721.903453370921797No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4115931.7520061258258908No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4103161.7465703875536662No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT3964321.6874711000269913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3001291.277543219972154No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2910761.2390077943038316No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT2603521.108226570595278No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2279770.9704176226209121No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2221260.9455119807800467No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1943970.8274794149613227No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1908620.8124321676689864No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1654920.7044410322215836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1647150.7011336174701989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1507720.641783187768065No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1476580.6285279888802758No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1377900.5865233958729849No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1169030.4976148091134302No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1159230.4934432950125846No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1158120.49297080719095815No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT970420.41307354221863846No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG954710.40638634971616033No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGT911700.3880785107898979No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC892190.37977379241158166No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT875510.37267370514606063No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC860670.3663568409362087No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACA808770.34426484278989333No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT732830.311939865155381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC687590.29268279393882407No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA656070.27926584246345104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA654590.2786358587012825No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCAGATCGGAAGAGCA650300.2768097571204021No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC649340.2764011190044009No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG627390.267057778747915No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG602800.25659068367242566No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTC559070.23797636615916226No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG551250.23464766817256907No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT549980.2341070739982758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT542300.23083796907026616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACG540420.23003771942643045No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA538200.22909274378317765No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC531500.22624079026525254No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA519050.22094126469836187No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG518160.22056242311165242No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC508760.2165611748924739No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA473410.2015139276001377No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT470010.20006666760596678No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT460290.19592920668145453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC459570.19562272809445364No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA458860.1953205061544944No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG455310.19380939645469827No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG453530.19305171328127937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG432370.18404464814108606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC423110.18010299298049107No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC415510.17686794122881483No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG410980.17493968011893413No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT407530.1734711368895548No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC402570.17135983995688187No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG373500.15898576700672026No Hit
TTTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC364960.15535059043312618No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA360780.15357131196970425No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA358020.15239647738620077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG351120.1494593909274421No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCAGATCGGAAGAGCACACGTC339230.14439823759488543No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG337830.14380230700905036No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT336150.14308719030604822No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACAGATCGGAAGAGC333710.1420485684278785No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC331920.14128662860741792No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCGAGATCGGAAGAGCACACGT327920.13958396979074622No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACAC327510.13940944726203736No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC320160.1362808116864031No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT314600.13391411593122943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA313720.13353953099156166No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG310280.13207524440922402No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT307300.1308067635908036No Hit
GTCCCCCGCCTCCCCTCCGCGCGCCGGGAGATCGGAAGAGCACACGTCTG292010.124298350264076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC286460.121935911155944No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT280050.1192074004022276No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAA279680.11904990446168548Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC272490.11598937523871809No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCAGATCGGAAGAGCACACGTCT247510.1053562709286033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT238130.10136353600350816No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT235260.10014187830254621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)