FastQCFastQC Report
Tue 11 Jul 2023
SRR8363861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8363861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20998427
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7515303.5789823685364626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC5009212.385516781804656No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2167101.0320296848902062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA2014480.9593480502134756No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1978620.9422705805534862No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1469420.6997762260954118No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1436510.6841036235714227No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1427690.6799033089478559No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1081550.5150623901495097No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1038360.4944941828261707No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1001080.4767404720363102No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT963280.4587391236495953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA926500.44122352593363307No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG907110.43198950092785526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT895520.42647003987489157Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACA887640.4227173778302537No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC843570.40173009149685357No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTC842760.4013443483171382No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC789760.3761043624839137No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC786030.37432803895263206No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT779840.3713801990977705No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC765010.3643177653259456No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA738760.3518168289462825No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC693440.33023425992813654No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG612630.291750424924686No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT582080.2772017161094971No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA570720.27179178707052676No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC544430.2592718016449518No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC536420.25545723020110034No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG532620.2536475708394729No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC503530.23979415220006717No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT493780.23515094725904945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC480750.22894572055325857No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC475520.22645505780028188No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCAGATCGGAAGAGCACA464070.2210022684080098No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT455600.21696863293617186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG423210.20154366800903706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT388080.18481384343693935Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG371050.17670371213996172No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG369700.17606080684043618No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT361090.1719604997079067No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT359770.17133188119281506TruSeq Adapter, Index 12 (100% over 48bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG351230.16726490989063134No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGAGATCGGAAGAGCACA347100.16529809590023098No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAGATCGGAAGAGCAC346140.1648409187983462No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT344270.16395037590196637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC339720.16178354692949143No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGGAGATCGGAAGAGCACA321650.15317814043880523No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC315830.1504065042586285No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT310620.14792536602860776No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG308690.14700624956336014Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC299980.142858319816051No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC292370.13923423883131816No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT291150.1386532429310062No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAA289960.13808653381512814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG288980.1376198321902874No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT286240.13631497254532446No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG282930.13473866399611742No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA275880.13138126965415076No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG275140.1310288623047812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT250640.11936132168376232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGCAGATCGGAAGAGCACACGTCT250600.11934227263785044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACA249060.11860888437024354No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG248420.11830409963565365No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC247560.11789454514854851No Hit
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT242380.11542769370296166No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC237570.11313704593205957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC235150.11198457865439158No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC227430.10830811279340115No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG223690.10652702700064154Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC222120.10577935194860072No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCAGATCGGAAGAGCA220520.10501739011212602No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCAC220040.10478880156118361No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC217140.10340774573257319No Hit
GTCCCCCGCCTCCCCTCCGCGCGCCGGGAGATCGGAAGAGCACACGTCTG213750.10179333909154244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACA213540.10169333160050512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)