FastQCFastQC Report
Fri 14 Jul 2023
SRR8392707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8392707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60450688
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length35-81
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA16493902.7284883837881213No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA11189891.8510773607737931No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAA10263841.697886383030082No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA7418851.2272565036811491No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA6863601.1354047781887942No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAA5613220.9285618056158434No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA5230220.8652043794770377No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAA4436280.7338675781489865No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAAA3886620.6429405733148975No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA3084920.5103200810551569No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAAA2897110.4792517828746631No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAAAAAA2728950.4514340680456772No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAA2451240.40549414425192315No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA2377950.3933702127591997No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAAAAAAAAA2102150.34774624897569406No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAAAAAAAAA1932080.31961257413645977No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAAA1897990.31397326693783867No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1875090.3101850552966411No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAAAA1858870.30750187657086714No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAAT1739380.2877353521600945No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAAAAAAAA1573010.2602137464506607No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAAAAA1567930.2593733920778536No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAA1507420.2493635804442788No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAA1378260.22799740509156818No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAAAAA1260400.20850052194608606No Hit
TCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAA1177090.19471904108022725No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAA1166380.19294734908558858No Hit
TGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAA1095270.18118404210718No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAAAAAAAAAA1075180.17786067215645254No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAAAAAAAAAA1064230.1760492783804214No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAA1032060.17072758543293998No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAAA1011290.16729172710160056No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAAAAAAAAA1001420.1656589913418355No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAAAAAAAAAA980730.1622363669376269No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAAAAAA943020.15599822453633613No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAAAAAAAAAAAAAAAAA942690.1559436345869215No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAAAA871360.14414393430890315No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAAAAAAAAA814480.13473461211888937No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA808710.13378011512457888No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAAAAAAAAA807990.13366100978040152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAT788240.13039388401998006No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA773670.12798365504127926No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAAAAAAAAAAAAAAAAAAA740950.12257097884477344No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA732200.1211235180648399No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAA731100.12094155156679111No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAATG721540.11936009727465799No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAAA718570.11886878772992625No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGAAAAAAAAAAAAAA715900.11842710541193509No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAAAAAAAAAAAAAAAA689650.11408472307213444No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA686930.11363476954968652No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAAAAAAA666610.11027335205845797No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA629530.10413942683332239No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAAAAAAAAAA627680.10383339226842216No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCAAAAAAAAAAAAAAAAAAA614340.10162663491935774No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAAAAAAAA613570.10149925837072359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)