FastQCFastQC Report
Mon 17 Jul 2023
SRR8392711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8392711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133535948
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length50-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA54777764.102098410234823No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA49528093.708970561245426No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA23712911.7757697724960175No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA20347641.5237574828914235No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA14768451.10595313256023No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA9578340.7172855057725729No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAA9296730.6961968023771397No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAAAAA7719690.5780982660938611No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA7123400.5334443725969579No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAA6762300.506402964990371No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA6496770.4865184317259649No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAAA6351610.4756479506177617No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA6146310.4602738133105551No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAAAAAA5727320.42889724345986596No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA5485920.41081971425402247No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA5170070.38716690729600395No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAAAAAAAAA4834850.36206355460179157No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAAAA4746180.35542339505464104No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAA4514760.338093230146537No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAA4501410.3370934993474566No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAAA4135270.30967466528189097No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA3873140.2900447451048912No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAA3791840.2839564968677947No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAAAA3665830.27452008653130616No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAAAAAAAAA3595920.26928479213702067No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAA3348930.2507886490610004No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAA3331890.24951258817588204No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAAAAAAAAA3171440.23749709703637256No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAA3166180.23710319561291468No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAAAA3090680.23144928734845244No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAAA3088930.2313182364946404No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAAA3010790.22546662865642741No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA2987890.22375173462654419No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAAAAAAAA2955050.22129247174700853No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAAA2943780.220448504248459No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAA2800870.20974651709515704No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAAAA2714710.203294321915474No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAA2668910.19986453385570754No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAAAAAA2605520.1951174974996246No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTAAAAAAAAAAAAA2591840.19409305425382534No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA2576580.19295029080858436No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGAAAAAAAAAAAAAA2441590.1828414023765346No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA2296300.17196118606204824No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAAAAAAAAA2111200.15809975003884347No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAAAAAAAA2082100.15592056155545472No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAAA1937970.1451272132354952No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAAAAAAAAAAAAAA1912180.14319589808131664No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTAAAAAAAAAAA1879910.14077932033702265No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA1836320.13751503078407024No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAAA1806600.13528941285533091No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAAAAA1798550.13468657892779554No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAAAAAAAAAA1793160.13428294229805446No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAAAAAA1741730.1304315449200241No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAAT1601610.1199384902707996No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAA1567710.11739984801695497No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAAAAA1554450.11640685697607059No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAAAA1547990.11592309210999874No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCAAAAAAAAAAAAAAA1497690.11215631614042984No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAAAAA1461150.10941997431283448No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAAA1414790.10594824997984813No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAAT1388240.10396002131201405No Hit
TCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAAAAA1359790.10182950886004119No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAAAA1340780.10040592215663158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)