FastQCFastQC Report
Wed 23 Aug 2023
SRR8434774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8434774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152719884
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1809342911.847461198962147No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG60738283.9771035970666397No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACG41625732.7256260880868663No Hit
GTGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTC32376672.120003574649127No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCT26562481.739294144566008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTG20411151.3365090036343925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGATTGGGGGGGCGGCCAAAGCCCGGGAGATCGGAAGAGCACACGTCT18379311.2034654243189447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTC13597260.8903398590847541No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACAC13303610.8711118455275936No Hit
CTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAA13224230.8659140940678033Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGTC11916160.7802625098903296No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAATAGCACACGT10292390.6739390923057537No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACGT9046430.5923544310706784No Hit
CTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTGA7096010.4646421811058997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGA6868230.44972729287824764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
NGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT6569070.4301384880569972No Hit
TACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACAC6197560.4058122516646228No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACG6176020.4044018262874008No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTC6040030.3954972883557193No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4663870.30538721467336893TruSeq Adapter, Index 7 (100% over 49bp)
AGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4659680.30511285616220085No Hit
CCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCT4622640.30268750073173184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGA4553730.29817531815307036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGATTGGGGGGGCGGCCAAAGCAGATCGGAAGAGCACACGTCTGAACT4383100.28700257525077744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGAA4096450.2682329172015348Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4096320.2682244048849592No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGT4046210.26494323424184896No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATG3991050.261331392839455TruSeq Adapter, Index 7 (97% over 49bp)
TTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAA3874180.2536788202379724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC3809690.24945605642288202No Hit
TGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTCT3597840.23558425437253477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT3501260.22926025795043165No Hit
ACTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTG3484560.22816675266725583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCTGAAGAGCACACG3472420.22737183325780946No Hit
CCGGGAACAGCCCAGCCTTAGAATCGGGCGAGATCGGAAGAGCACACGTC3438820.22517172681980296No Hit
TGGCCGAGGGCACGTCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG3406550.22305870792830093No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGATGAGCACACGT3392360.22212955583439287No Hit
CGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGA3233220.2117091707586682Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGT3210680.2102332660231722No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG3107230.20345942640972672No Hit
TCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG3035490.1987619372471498No Hit
GACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTG2994410.19607204520925384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2855660.18698678424873608No Hit
GAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCTGAACT2751650.18017627619465712Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACG2722300.1782544570293152No Hit
TTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG2634960.17253548987766387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACGT2603090.17044866272947143No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT2395400.15684925480954398No Hit
GCATTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGT2374900.15550692796492696No Hit
GTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAA2287540.14978665122611015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
NCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2285780.14967140755554792No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG2228420.14591551156495117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGT2194470.1436924873515488No Hit
TTACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCT2005600.13132540095433806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACGT2005220.13130051879819396No Hit
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA1929400.12633587385385914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1897160.12422481934310531No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACG1869540.12241627946757737No Hit
GAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACTC1867880.12230758373284255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGATCACACGT1859590.12176475985275106No Hit
GGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACT1805360.11821381425355196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGTAAGAGCACACG1762080.11537986762745316No Hit
ATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGA1744290.11421498984375865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG1726670.11306124355097075No Hit
CGCCAGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1681490.11010288614415134No Hit
TTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAAC1675640.10971983189824842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAAGATCGGAAGAGCACAC1668690.10926475035824411No Hit
TACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTG1636670.10716810130631058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCTG1615900.10580809503495955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGCGTGGATTGGGGGGGCGGCCAAAGCCCGGGAGATCGGAAGAGCACA1591920.10423790002354899No Hit
CGGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC1552530.10165866810113607No Hit
TTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGA1551230.10157354493537986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGTGCACACGT1545710.10121209887770737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)