FastQCFastQC Report
Wed 23 Aug 2023
SRR8434775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8434775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140620485
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1778611812.648312228477948No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG63256264.4983673609147345No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACG44748263.182200658744706No Hit
GTGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTC32374272.302244228499141No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCT28424262.0213456097808224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTG20414731.4517607445316378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGATTGGGGGGGCGGCCAAAGCCCGGGAGATCGGAAGAGCACACGTCT15933321.1330724680689304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTC14094951.002339737343389No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACAC12946390.9206617371572854No Hit
CTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAA11052510.7859815019127547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGTC10992700.7817282097981669No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAATAGCACACGT10588230.7529649751954702No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACGT9775980.6952031206548605No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACG7226380.513892410483437No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTC7217360.513250967666624No Hit
NGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT6415520.4562294035609392No Hit
ACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGA6292060.4474497438975552Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCT5649720.4017707661867331Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5635760.40077802320195377TruSeq Adapter, Index 1 (100% over 49bp)
CTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTGA5564530.39571261612417286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACAC4767400.33902599610575945No Hit
AGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4586310.32614807152741654No Hit
GTGGATTGGGGGGGCGGCCAAAGCAGATCGGAAGAGCACACGTCTGAACT4535870.3225611119176555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGATGAGCACACGT4411790.3137373619497899No Hit
GGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4051720.28813156205513013No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGT4036660.28706059433659326No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCTGAAGAGCACACG3746290.2664113980263971No Hit
CCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGAA3628860.25806055213079376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGT3562340.2533300891402842No Hit
TGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT3480440.24750590214505375No Hit
CGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGA3350540.23826827222221572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGA3306240.23511794885361118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTCT3273660.23280107446649753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTG3188410.2267386576002778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC3182140.22629277661785907No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACG3176400.22588458573443265No Hit
TCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG3072100.21846745870631865No Hit
ACTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTG2958260.21037190989634263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2957360.21030790784145No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG2900670.20627648951715674No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACGT2895570.20591381120609845No Hit
CCGGGAACAGCCCAGCCTTAGAATCGGGCGAGATCGGAAGAGCACACGTC2637450.1875580218628886No Hit
TTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG2635450.1874157950742383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGT2633920.1873069915809208No Hit
TTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAA2552760.18153542849749096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAA2393440.17020564251360673Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
NCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2371520.16864683690999926No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG2338380.16629013902206355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT2284510.16245926046976725No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACG2271620.16154260881691598No Hit
GCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2167420.15413259312823446No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACGT2165880.15402307850097374No Hit
GAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCTGAACT2112730.15024340159259159Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCT2047000.1455691181835989Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGATCACACGT2023730.1439143094976525No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGATGAGCACACG2001120.14230643565196066No Hit
CGGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC1991110.14159459057476584No Hit
TGGCCGAGGGCACGTCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG1940290.1379806078751613No Hit
GCTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGT1886840.13417959694848158No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGTAAGAGCACACG1866440.13272888370424835No Hit
GCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG1780160.1265932200418737No Hit
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA1709370.12155910285759573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGTGCACACGT1698160.12076192170721073No Hit
TTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAAC1647930.11718989591025804Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACT1610700.1145423442395324Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAAC1607830.11433824879781918Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
NGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACG1603320.11401752738941272No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAAGATCGGAAGAGCACAC1565760.11134650829855977No Hit
CGCCAGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1548790.11013971399686183No Hit
GTGGATTGGGGGGGCGGCCAAAGCCCGGGCGAGATCGGAAGAGCACACGT1503250.10690121001929412No Hit
TTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGA1456160.10355248028052243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACTC1431310.10178531243154225Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGA1423070.10119933806230295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTG1419330.10093337396752686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGATCACACGTCT1411210.10035593320560655Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)