FastQCFastQC Report
Fri 14 Jul 2023
SRR8434776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8434776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156110600
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1878084112.030471345315437No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG59580803.816576196619576No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACG46436382.9745821231870226No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCT30693471.966136188061541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTC28650771.8352866493370725No Hit
CCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTG21797271.3962709771149429Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGATTGGGGGGGCGGCCAAAGCCCGGGAGATCGGAAGAGCACACGTCT15899741.018492017838635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTC14849260.95120126371944No Hit
CTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAA13722120.8789998885405603Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACAC12157870.778798492863393No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGTC11636730.7454157501156231No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAATAGCACACGT10527850.674384058481615No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACGT9612740.6157647206531779No Hit
CCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTC7816660.5007129560708882No Hit
ACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGA7495930.4801679065995519Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATTTATCTCGTATG7336940.4699834604440698TruSeq Adapter, Index 2 (97% over 49bp)
CTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTGA7259480.4650215936650042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
NGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT6871160.44014692147746537No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACG6185350.3962158879666082No Hit
CCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCT5990480.3837330712968882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGATTGGGGGGGCGGCCAAAGCAGATCGGAAGAGCACACGTCTGAACT5105970.32707388223477457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4884760.312903800254435No Hit
TACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACAC4577020.2931908531515477No Hit
GGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT4286730.27459570330265853No Hit
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC4282680.2743362718482922No Hit
CCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGAA4218040.27019561772230716Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGATGAGCACACGT4165220.26681211910017644No Hit
ACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTGA4131320.2646405817414064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGAGATCGGAAGAGCACACGT3950410.25305200287488483No Hit
ACTTATTCCGTGAATCGGAAGCGGGGCAAGATCGGAAGAGCACACGTCTG3853660.2468544736872448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCTGAAGAGCACACG3829230.2452895575316474No Hit
TGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT3728060.23880889574442737No Hit
GCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGT3689330.23632796235489453No Hit
GACCAGCGTGGATTGGGGGGGCGGCCAAAGATCGGAAGAGCACACGTCTG3676220.23548817312853834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACTC3528440.2260218076158826Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGA3524540.2257719847339002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAA3445650.22071851623144106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA3351370.21467920820238984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG3176080.20345063051451984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG3148690.20169610519721273No Hit
TCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG3120080.1998634301578496No Hit
TGGATTGGGGGGGCGGCCAAAGCCCGGGCAGATCGGAAGAGCACACGTCT2973530.19047585493874214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACGT2882740.18466010636049057No Hit
CCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2856770.18299654219508477No Hit
GCATTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGT2831430.1813733340336915No Hit
GAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCTGAACT2791500.17881553206508718Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTATTAGGCGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG2768770.17735951306317443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGTAAGAGCACACG2743930.1757683334763943No Hit
TTACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCT2717680.17408683330920513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGAACAGCCCAGCCTTAGAATCGGGCGAGATCGGAAGAGCACACGTC2716950.17404007159027No Hit
GCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2688070.17219010112061575No Hit
GTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAA2650410.16977770888075505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT2637470.16894880936976733No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2630320.16849080075279962TruSeq Adapter, Index 2 (100% over 49bp)
TTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAAC2549180.1632932036645814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGT2467740.1580763894315953No Hit
TGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAAC2285880.1464269562733088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
NCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG2270680.14545328760506973No Hit
GGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGTCTGAACT2257400.1446026086633451Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCTGAAGAGCACACGT2070350.13262071890057434No Hit
CGCTCTGTATACATTAGCATGGGATAACATTAAGATCGGAAGAGCACACG2055830.13169060909380914No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGATCACACGT1968950.12612532396903223No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGTAGAGCACACG1960970.1256141479182067No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGTAAGAGCACACG1950740.12495884328162213No Hit
ATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGA1895940.12144851150402343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTTATTCCGTGAATCGGAAGCGGGGCAGATCGGAAGAGCACACGTCTG1828240.11711184250140605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC1825520.11693760705551064No Hit
GCGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACG1724610.11047360012708939No Hit
NGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACG1702590.10906306170112728No Hit
ACGCCCGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGATGAGCACACG1662670.10650590030401523No Hit
CGCTCTGTATACATTAGCATGGGATAACATTAGATCGGAAGAGCACACGT1651780.10580831794894134No Hit
CGCCCGTGGAACGCCGTCTCCCCGATTGTGGAAAGATCGGAAGAGCACAC1644540.10534454418854323No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATTTTTCTCGTATG1616400.10354197600931647TruSeq Adapter, Index 2 (97% over 39bp)
CGTGGAACGCCGTCTCCCCGATTGTGGAAGATCGGAAGAGCACACGTCTG1615760.10350097943381167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCAGTGGAACGCCGTCTCCCCGATTGTGGAGATCGGAAGAGCACACGT1611960.10325756226675191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)