FastQCFastQC Report
Tue 26 Sept 2023
SRR8443308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15646219
Total Bases782.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA5291943.382248452485549No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC3335622.1319016434577582No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA3212962.0535057063946245No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3018291.9290858705224567No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2920291.8664509297741518No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA2711851.7332302455947985No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2133081.3633197899121827No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2015271.288023643284042No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG1839901.175939055946999No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1777891.1363064776224852No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC1658041.059706501615502No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1495040.9555279777178115No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1437140.9185222321124356No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1428700.9131279576235No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1311240.8380555072123175No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1201950.7682047656369887No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1130390.7224684762497572No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1105360.7064710010770014No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1065140.6807651100882584No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG1050310.6712867818097139No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1047860.6697209082910063No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG982300.6278194111944874No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA890390.5690767846212558No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA870100.5561087953581628No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG789610.5046650567782542No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG776190.4960879046880272No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC775620.4957235994204095No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG759030.4851203987365893No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA747220.4775722492443702Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA712190.45518345358709345No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT693900.4434937284209048No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA675620.43181039457520054No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA653210.41748744536938925No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA643290.41114725544874453No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC637770.407619246541289No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG594740.3801173944963956No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA590860.37763756214840144No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA562520.35952455989526927No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC500290.3197513725200957No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA498870.3188438050112938No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG497730.3181151944760584No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC481980.30804886471293796No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA462290.29546435467891635No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA456770.2919363457714608No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG456080.2914953446580289No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG449970.2875902478419866No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG449310.28716842069000825No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC447150.2857878954653517No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG424810.27150968550293203No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA424620.27138825041372616No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA424130.2710750757099846No Hit
TGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAG419860.2683459818630942No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA408270.2609384414215345No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG404310.2584074785096642No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC385960.24667940542056838No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG385690.2465068397674863No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC384420.24569514206595217No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG384410.24568875074546764No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC379750.2427103953996809No Hit
TCGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG369130.23592281304511972No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG368630.23560324702089366No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA358180.22892431711456934No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA336510.21507432562461257No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC333150.21292684194181352No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA326900.20893226663898798No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT324860.20762843726014574No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA316810.20248342427010643No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT312240.19956259080868036No Hit
TCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC308520.1971850195884386No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC303130.19374009784728183No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA299740.19157344020302924No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA289010.18471555332313833No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC288200.18419785636389213No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG279340.1785351464146066No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA278040.1777042747516189No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG277070.17708431666462038No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG273540.1748281805335845No Hit
GCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA269200.17205434744330242No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA265690.1698109939532356No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG263460.16838572948518746No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG263250.16825151175501252No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT258480.16520285188389602No Hit
TGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCG253330.16191132183436777No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG250080.1598341426768985No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG248610.15889461856567394No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG242600.15505343495447688No Hit
TGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG241280.1542097806505201No Hit
AGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCA238640.1525224720426066No Hit
TTGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA236310.15103329436971322No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC232590.14865572314947145No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG232180.1483936790096061No Hit
AAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGA228410.14598415118694172No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT223100.1425903600096611No Hit
GCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC221200.14137600911760217No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC217470.13899204657687586No Hit
TCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG213110.13620543084562475No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC209640.133987642637496No Hit
TGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCGG209110.1336489026518164No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA204940.13098372200977118No Hit
TTGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA204550.13073446051087487No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC198580.1269188421816159No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC190800.12194639484465863No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG188680.12059143490194021No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC179680.1148392464658714No Hit
TTACCCTACGCCGGCAGGCGCGGGTCTGTAGGCACCATCAATAGATCGGA172960.11054427910027337No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG171120.10936827613112152No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA169380.10825618636681489No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA167280.10691400906506551No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG165270.1056293536476768No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG165050.10548874459701733No Hit
TAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA163900.10475374274129745No Hit
TTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAG163290.10436387219174166No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA162320.10374391410474314No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA161100.1029641730056316No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA160380.10250399793074608No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG158640.10139190816643945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)