FastQCFastQC Report
Tue 26 Sept 2023
SRR8443309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14709086
Total Bases735.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA5613663.8164573923899825No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA4418953.0042315341687447No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC3219622.188864760189722No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3025982.057218239121044No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2722701.8510327562161237No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2590491.7611495370956427No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1871461.272315628584944No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1641631.1160652674136244No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1630741.1086616802702765No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG1473941.0020609030363952No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1467970.9980021872195186No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1315760.8945219301865527No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1201850.8170800007559953No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1160260.7888049604169831No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA1092220.7425478374387096No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG1055780.7177740343621622No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA1014620.6897913303382684No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA987010.6710206195000832Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA976170.6636510249515164No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT972020.6608296395846758No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC965900.6566689459834554No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG950100.6459272860325924No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG900860.6124513786920547No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA880770.5987931541089637No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG835480.5680026617561418No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA796670.5416176096869649No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC696810.47372759938992814No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG686410.466657139675436No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT626300.4257912422294628No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC618090.4202096581663877No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG600950.40855699667538825No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA597570.40625909726817827No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC586670.39884871160587404No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG564950.38408232843291557No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA563790.3832937002339914No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA557960.3793301636825021No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG523090.35562372808208476No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA505550.3436991258328356No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC495230.3366830542699934No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC477370.32454089941414443No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG477290.3244865112624945No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA473650.32201185036242225No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA471300.32041419840770524No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG463530.3151317491787049No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG458490.31170529562475874No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA446570.3036014610289178No Hit
GCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA433550.29474978934789015No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA420870.2861292673113748No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC410880.27933754687408857No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC404080.2747145539838437No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG401910.2732392753703391No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA395180.26866387211278797No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC384550.26143704646230226No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC375750.255454349780809No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG370890.2521502695680751No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA370090.25160638805157576No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT364240.24762925446217393No Hit
TCGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG350420.23823370126464688No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC335080.22780477318577103No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG333720.22688017460772203No Hit
TTGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA328890.223596489951857No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA325720.22144135944272814No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG316070.21488078864995416No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG302860.20589994510875792No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG300310.20416632277491611No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC297850.2024938871116805No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA295290.2007534662588824No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT294090.19993764398413333No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA271220.18438943113120693No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC270800.18410389333504473No Hit
TTGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA269320.18309771252952087No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA268000.18220030802729686No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA262220.17827076407058876No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG260570.17714900844280876No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG257040.17474913125125519No Hit
ACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA254860.17326705411879434No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA254190.17281155334872608No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG253790.1725396125904764No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC251370.17089437100306573No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT241600.1642522179828169No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG241240.16400747130039217No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC239090.16254578972480005No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC235680.16022749476072137No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG234250.15925530654997871No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG233460.15871822355243556No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG232650.15816754351697992No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG227200.1544623506858278No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG227100.15439436549626537No Hit
TGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG225600.153374587652829No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC223120.1516885549516809No Hit
TCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG221970.150906725271713No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT216870.14743948060402937No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA207800.1412732239107175No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA206890.14065455868569943No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT204500.13902971265515748No Hit
TTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAG190060.12921265128234347No Hit
TCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA189170.12860758309523787No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG185350.12601054885395327No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA181720.12354268647283727No Hit
TGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCGG177640.12076889073869036No Hit
ACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA174320.11851178244521787No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA172820.11749200460178151No Hit
AAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGA168610.11462982812120345No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC166810.11340609470907982No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG163130.1109042397331826No Hit
ACGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA157410.10701548689021194No Hit
TCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC151160.10276641254256043No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA150940.10261684512552309No Hit
TTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC150330.10220213546919231No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG150040.10200497841946128No Hit
TGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGG149380.10155627616834928No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC148110.1006928642609065No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA147510.10028495312353194No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG147370.10018977385814454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)