FastQCFastQC Report
Tue 26 Sept 2023
SRR8443310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18574469
Total Bases928.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA6223083.3503407284482805No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4774002.570194604217219No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC4226812.275602064317424No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA3528661.8997366761870824No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3080191.6582923581826214No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2722021.4654631580585158No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG2112251.1371792108834982No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2064231.111326520289759No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1810770.9748703987177239No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1670780.8995035066682122No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1614450.8691769331333241No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1553970.8366161099948538No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1479120.7963188611206059No Hit
ACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1453590.782574188258087No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1383340.7447534570167255No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1377390.741550135295927No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1302210.7010752232001894No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA1214710.6539675508355044No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1105970.595424827487666No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1054380.5676501438614476No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG1025230.5519565592965269No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1016230.5471111987104449No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG1010920.5442524359646567No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC920190.4954058175229666No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA907390.48851463802276124No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG880990.4743015803035877No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA865220.4658114318099753No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA864370.4653538144212898No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG840140.45230902697676045No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC818560.44069092903813295No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA812250.43729379289389103No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA781690.4208411018371508No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG756680.40737638314182767No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT708200.3812760407847998No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA692660.37290971817283175No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC672650.36213686646977633No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA654940.35260227358316404No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG653690.35192930683509716No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC651560.3507825714963911No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC639110.34407982268564447No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC631380.33991819631559855No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC628700.3384753556077431No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG616700.33201487482630054No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC611640.3292907054301256No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610110.3284669941304917Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT597880.32188268746740484No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC589450.31734419971844147No Hit
TAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT552430.29741361650769127No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG548440.2952655066478616No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA543410.2925574884536403No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG532260.2865546250608833No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG515110.27732152127740506No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA482060.2595282804585154No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG472340.25429529102554693No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC470310.2532023930266863No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA463910.24975680327658356No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC450650.2426179720130896No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG448850.2416488998958732No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA442050.2379879607863891No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG440900.23736883137816753No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA437870.23573755998085327No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC433660.23347100797336387No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA415090.22347341396408157No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA409520.2204746741346953No Hit
TCGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG395570.21296436522626835No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC394470.21237215448796945No Hit
TCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA382720.20604626705614035No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT373600.20113630166224403No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA367620.19791682873949182No Hit
TTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC360330.19399208666476547No Hit
GCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA357860.19266230437058524No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG355370.19132175460843592No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA346900.1867617319235344No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA344740.18559884538287474No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA335850.18081270587062273No Hit
TCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG331780.17862152613891683No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC320630.17261866274615978No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA318580.17151499727933003No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC306590.16505990023187203No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT300420.16173813636341366No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA300020.16152278700403225No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG296560.159660015045383No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC292620.15753882385547602No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG290450.15637055358083185No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC288860.1555145398772907No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG285640.1537809775342703No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG283380.15256425365376527No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG281710.15166517007834787No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC280670.1511052617439562No Hit
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC280640.15108911054200258No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC277430.1493609319329667No Hit
TGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG265000.14266895059018914No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG259800.13986940891823071No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC259620.13977250170650907No Hit
TAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT255340.13746826356112793No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC253090.13625692341460743No Hit
TCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC251450.13537399104114362No Hit
TTGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA248550.13381270818562835No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT246750.13284363606841196No Hit
GCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT242970.13080858462225758No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG240780.12962954687964431No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG240410.1294303487222165No Hit
TTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC236540.1273468436702013No Hit
TTGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA227950.12272221617748535No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA225520.12141396881924323No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG224720.1209832701004804No Hit
ACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA223310.1202241636086609No Hit
TTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAG214170.1153034307467955No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG213080.11471660374248116No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA209560.11282152937992468No Hit
TTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC205320.11053882617048164No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA204500.11009735998374975No Hit
ACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG202290.10890755477316741No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC199450.10737857432155933No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT199410.1073570393856212No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA197640.10640411847035842No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA196360.10571500052033789No Hit
TTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA195350.10517124338789981No Hit
TGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCGG190110.10235016678000323No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA189980.10228017823820429No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA187980.1012034314412972No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG186390.10034741773775606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)