FastQCFastQC Report
Tue 26 Sept 2023
SRR8443311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16699706
Total Bases834.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA4814472.8829669216931126No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3410272.0421137952967556No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3362532.0135264656755036No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC3260001.9521301752258393No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3179001.9036263273137861No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2417061.4473667979544071No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2218691.328580275604852No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2178561.3045499124355842No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA2043741.2238179522441892No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1680591.0063590341051512No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1540880.9226988786509176No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1424280.8528772901750485No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1266580.7584444899808416No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG1194790.7154557092202701No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1191930.7137431042199186No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1169410.7002578368744935No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1126960.6748382276909546No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1076850.6448317114085721No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG1042220.6240948193938265No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1036570.6207115262987265No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA994290.5953937153145091No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA977400.5852797648054403No Hit
ACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA894430.5355962554071311No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG855850.5124940522905014No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC813710.48726007511749014No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC704720.42199545309360537No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG676320.40498916567752746No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG669910.40115077475016625No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG665560.3985459384734078No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT655480.39250990406657454No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG650340.38943200556943935No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC634880.3801743575605463No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG608110.36414413523208133No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG603790.36155726334343846No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG586850.35141337218751034No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA586750.3513534908937918No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA552480.330832171536433No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC546660.3273470802420114No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA539180.32286795947186137No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA529730.31720917721545516No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA521960.3125564006935212Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA520060.3114186561128681No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA509290.3049694407793766No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC482370.28884939651033376No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA475740.28487926673679165No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA457070.27369942919953205No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC456150.27314852129732103No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT454620.27223233750342674No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC453160.2713580706151354No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC451020.2700766109295577No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA448970.2688490444083267No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC433930.25984289783305164No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG429280.25705841767513754No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG420430.2517589231810428No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG418620.25067507176473647No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC402110.24078867017179825No Hit
TAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT392840.2352376742440855No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA370810.22204582523788144No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA346690.20760245719295897No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG331770.19866816817014624No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC327420.19606333189338782No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA327390.19604536750527224No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC321060.19225488161288587No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT314710.1884524194617558No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT313320.18762006947906748No Hit
TCGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG310360.1858475831849974No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG305200.18275770842911845No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG293870.1759731578508029No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG291920.1748054726232905No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG289060.173092867622939No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT288250.17260782914381845No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA274240.16421845989384484No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA269380.16130822901912167No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG266520.15959562401877014No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG265390.15891896539975015No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG265220.15881716720042854No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC255020.15270927524113298No Hit
TCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA254640.15248172632500237No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC253640.15188291338781654No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA251190.15041582169171122No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC251160.15039785730359564No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG249860.14961940048525404No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC233530.1398407852210093No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT225840.13523591373405017No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA224360.1343496705870151No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG223600.1338945727547539No Hit
TGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG222770.13339755801688966No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC222410.13318198535950274No Hit
GCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA220310.13192447819141245No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA220050.13176878682774415No Hit
TTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC220030.13175681056900043No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA214820.1286369951662622No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG213190.1276609300786493No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC210170.12585251500834804No Hit
TAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT209630.1255291560222677No Hit
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC204400.12239736436078574No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA199430.11942126406297213No Hit
GCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT198880.11909191694751992No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA196960.1179421961081231No Hit
TCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG195500.11706792921983178No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC191590.11472657063543515No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA188340.11278042858958116No Hit
TTGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC186200.11149896890400345No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA185860.11129537250536027No Hit
TTGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA182020.10899593082656664No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG177650.10637911829106453No Hit
TCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC175820.10528329061601444No Hit
TTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC171460.10267246620988418No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATC170980.10238503600003496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)